水产学报2012，Vol.36Issue(12)：1834-1841,8.DOI:10.3724/SP.J.1231.2012.27857
Flg22诱导的海带孢子体防御反应初步研究
Preliminary study on flg22-induced defense responses in sporophytes of Saccharina japonica (Phaeophyta)
摘要
Abstract
Flg22 is an N terminal 22 amino acid peptide of flagellin. Flg22-induced defense responses were investigated in sporophytes of Saccharina japonica in this study. Our results would enrich the theory of plant molecular pathology. The results of Evans blue dye showed that cell death in the sporophytes of S. japonica was not observed within 2 h after flg22 induction, whereas large amounts of cell death were observed from 4 to 10 h. By using TdT-mediated dUTP-biotin nick and labeling (TUNEL), fragments with 3'-OH groups of nuclear DNA were observed. The number of 3'-OH increased gradually with the induction time and spread from the induction sites. The concentration of H2O2 increased rapidly in flg22-induced sporophytes of S. japonica, with a peak of about 20 umol/L at 3 h by using Luminol-dependent fluorescence detection method. Histological observation of radical oxygen species (ROS) production, detected by using the fluorescent dye 2 \T- dichlorofluorescein diacetate (DCFH-DA), showed that green DCF fluorescence intensity increased gradually with the induction time, reaching the highest level at 3 h, then decreased from 3 to 5 h. The histological observation showed a consistent result with that of quantitative analysis of H2O2. The results indicate flg22 is an effective elicitor which could induce defense responses in sporophytes of S. japonica.关键词
海带/flg22/激发子/防御反应Key words
Saccharinajaponica/ flg22/ elicitor/ defense response分类
农业科技引用本文复制引用
王莎莎,魏晓娇,路博钧,王高歌..Flg22诱导的海带孢子体防御反应初步研究[J].水产学报,2012,36(12):1834-1841,8.基金项目
国家教育部回国人员科研启动基金资助[2010]-1561 （）
山东省自然科学基金(Y2007D42) （Y2007D42）
青岛市科委课题[12-14-1-(4)-jch] （4）
中国海洋大学"国家大学生创新训练项目"(201210423048) （201210423048）
