山东农业科学2012，Vol.44Issue(12)：28-31,4.
根癌农杆菌介导的玉米自交系A188愈伤组织再生条件的优化
Optimization of Conditions for Agrobacterium tumefciens-Mediated Transformation and Regeneration of Maize Inbred Line A188 Callus
摘要
Abstract
The factors affecting the Agrobacterium tumefciens - mediated transformation and regeneration of maize (Zea mays L. ) embryogenic callus were optimized, and the genetic transformation system was established for the maize inbred line A188. The results indicated that 1.0-1.5 mm immature embryos were easier to induce embryogenic callus; the addition of acetosyringone in infection solution and common medium could increase the transformation efficiency significantly; appropriate bacterial concentration ( OD600 = 0.4) , incubation time (3 days) and co -culture temperature (24℃) were benefit to increasing formation efficiency.关键词
玉米/根癌农杆菌/愈伤组织/再生体系Key words
Maize/ Agrobacterium tumefciens/ Callus/ Regeneration system分类
农业科技引用本文复制引用
仇平平,辛相启,刘文浩,苏文敏,竺晓平..根癌农杆菌介导的玉米自交系A188愈伤组织再生条件的优化[J].山东农业科学,2012,44(12):28-31,4.基金项目
国家自然科学基金(30871620)和国家转基因专项子课题(2009ZX08009-050B)资助. （30871620）
