山东医药2012，Vol.52Issue(47)：18-20,3.
人MEF2C基因shRNA慢病毒载体构建及其对Daoy细胞MEF2C mRNA表达的影响
Construction of shRNA lentiviral vector targeting human MEF2C gene and its influence on the expression of MEF2C mRNA in Daoy cell line
摘要
Abstract
Objective To construct a short hairpin RNA( shRNA) lentivirus vector of of human MEF2C gene, and to detect its interference efficiency on the expression of MEF2C mRNA in Daoy cell line. Methods The specific siRNA sequences targeting human MEF2C gene were synthesized and cloned into pGLV-3 lentiviral vector. The lentivirus particles were packaged and transmitted into Daoy cell line after screening for the valid siRNA. The expression of MEF2C mRNA was determined by Real-time PCR. Results PCR and DNA sequencing demonstrated that MEF2C-shRNA plasmids was correctly constructed. The Daoy cell line was infected with the packaged virus particles and the expression of MEF2C mRNA was inhibited about 81. 1%, compared with the negative control group. Conclusion The recombinant lentiviral MEF2C-shRNA vector has been successfully constructed and packaged into lentiviral particles targeting human MEF2C gene,which can effectively inbit the expression of MEF2C mRNA.关键词
肌细胞增强因子2C/短发卡RNA/慢病毒/Daoy细胞株Key words
myocyte enhancer factor 2C/ short hairpin RNA/ lentivirus/ Daoy cell line分类
生物科学引用本文复制引用
付鹏,刘爱华,王静波,杨宪勇,谭业克,刘兴田..人MEF2C基因shRNA慢病毒载体构建及其对Daoy细胞MEF2C mRNA表达的影响[J].山东医药,2012,52(47):18-20,3.基金项目
山东省自然科学基金资助项目(2009CM132). （2009CM132）
