山东医药2012，Vol.52Issue(47)：24-26,3.
氯胺酮对哮喘大鼠离体气管平滑肌的舒张作用及机制探讨
Effects and mechanism of ketamine on tracheal smooth muscle isolated from rats with induced asthma
摘要
Abstract
Objective To investigate the effects of ketamine on tracheal smooth muscle isolated from rats with induced asthma and explore its relationship with large-conductance Ca2 + -activated-K+ channel ( BKca) or protein kinase G(PKG) signal pathway. Methods Peritoneal injection and continuous nose-dripping with ovalbumin( OVA) in SD rats were used for making asthmatic model,2-3 isolated tracheal rings were made from each rats . The effects of ketamine in 10 2,10 ~' , 4 x 10 ~ and 10 x 10 ~ mg/mL on the tracheal rings pre-constricted by acetylcholine (Ach) were determined by cumulative addition method; Then the tracheal ring were pretreated with Ca2 + -activated-K + channel blocker (IBTX) or protein kinase G blocker( KT-5823) , the effect of 4 x 10"' mg/mL ketamine was measured. Results Ketamine in 10 "2,10 "',4 x 10 "' and 10 x 10 ~' mg/mL relaxed tracheal rings of asthmatic rats in a dose dependent manner , with the relaxation effect of (4.61 ±1.85)%, (14.02 ±3.96)%, (70.10 ±11. 12)%, 100%. Pretreatment to the rings with IBTX(3xlO"6 M) or KT-5823(2 x 10~6 M) attenuated the relaxation effect of Ket(4 x 10"1 mg/mL) , which was different compared with control group of(70. 10 ± 11. 12)% , P <0. 05. Conclusion The dilatation effects of ketamine on the isolated asthmatic rat tracheal ring are dose dependent. Themechanism may be related to Ca2 + -activated-K+ channel and PKG signal pathway.关键词
氯胺酮/钙激活钾通道/PKG信号通路/气管平滑肌Key words
ketamine/ Ca2 * -activated-K + channel/ PKG signal pathway/ tracheal smooth muscle分类
医药卫生引用本文复制引用
周述芝,马楠,廖常莉,杨艳,魏继承..氯胺酮对哮喘大鼠离体气管平滑肌的舒张作用及机制探讨[J].山东医药,2012,52(47):24-26,3.基金项目
四川省泸州医学院资助项目(09017). （09017）
