上海农业学报2012，Vol.28Issue(4)：42-45,4.
气孔保卫细胞大小与油菜单倍体及二倍体倍性的相关性研究
Correlation between the stomatal guard cell size and the ploidy level in haploid and diploid of Brassica napus
何婷 1刘成洪 2杜志钊 3高润红 3徐红卫 3邹磊 3王亦菲3
作者信息
- 1. 上海市农业科学院生物技术研究所,上海201106
- 2. 上海市农业遗传育种重点实验室,上海201106
- 折叠
摘要
Abstract
The width, length and perimeter of stomatal guard cells were measured in different leaves from seed-germinated diploid plants and microspore-derived haploid plants of B. napus Strain 2105,and the correlation between the stomatal guard cell size and the ploidy level of plants was studied. The results indicated that the width, length and perimeter of stomatal guard cells were significantly different between the haploid and diploid plants. The calculated perimeter of stomatal guard cell was 43 - 59 μm for haploid plants and 75 - 94μm for diploid plants. Compared with a single observed index of the width or the length, the stomatal guard cell perimeter calculated by the length and width had a wider range to distinguish the haploid from the diploid and could be used to rapidly identify the plant ploidy in the construction of double haploid population.关键词
油菜/气孔保卫细胞/倍性鉴定Key words
Brassica napus-,Stomatal guard cell/Ploidy identification分类
农业科技引用本文复制引用
何婷,刘成洪,杜志钊,高润红,徐红卫,邹磊,王亦菲..气孔保卫细胞大小与油菜单倍体及二倍体倍性的相关性研究[J].上海农业学报,2012,28(4):42-45,4.
