实验动物与比较医学2012，Vol.32Issue(6)：493-498,6.DOI:10.3969/j.issn.1674-5817.2012.06.008
uPA-/-小鼠肝纤维化模型中的a-SMA、MMP13表达分析与模型评价
The Expression of a-SMA, MMP13 and Model Evaluation in uPA Knock-out (uPA-/-) Mice with Liver Fibrosis
摘要
Abstract
Objective To evaluate the feasibility of uPA knock-out (uPA-/-) mice liver fibrosis model by analysing the expression of a-Smooth Muscle Actin (a-SMA) and Matrix Metalloproteinase-13 (MMP13). Methods Adult male C57BL/6J WT mice and uPA knock-out (uPA-/-) mice were divided into four groups with 10 mice in each group: control groups (Con-WT, Con- uPA-/-) and liver fibrosis model groups (Mod-WT, Mod-uPA'). Mice were intraperitoneally injected with 0.15ml 10% CCU (or olive oil as control) twice per week for 6 weeks to induce liver fibrosis. The mRNA expression of a-SMA and MMP13 was detected by real-time polymerase chain reaction (PCR) and the protein expression of a-SMA and MMP13 was analyzed by western blot and immunohistochemistry. Result The real-time PCR showed that the mRNA expression of a-SMA, MMP13 in uPA-/-mice wes significantly higher than that in WT mice. The western blot results indicated that there were no expression of a-SMA protein and MMP13 protein of the mice liver in the control group, a few expression of a-SMA protein in Mod-WT group, and a significant increase of a-SMA protein expression in the Mod-uPA-/-group. The immunohistochemistry results demonstrated that the protein expression of a-SMA and MMP13 of Mod-uPA-/-group was higher than Mod-WT group. Conclusion The mRNA and protein expression of a-SMA were significantly higher in the uPA-/- mice. The knocking-out of the uPA gene promoted the hepatic stellate cells transforming into myofibroblasts, thus speeding up the deposition of extracellular matrixin in the liver. Also, the protein expression of MMP13 in liver fibrosis model mice was significantly increased. uPA-/- mice are susceptible animal strain to establish liver fibrosis model.关键词
uPA基因缺失小鼠/肝纤维化/a-SMA/MMP13Key words
uPA knock-out mice/ Liver fibrosis/ a-SMA/ MMP13分类
生物科学引用本文复制引用
夏敏杰,成倩倩,王玉柱,田芳,温成丽,王晓东,李卫华,丁训诚..uPA-/-小鼠肝纤维化模型中的a-SMA、MMP13表达分析与模型评价[J].实验动物与比较医学,2012,32(6):493-498,6.基金项目
上海市科委基金项目(09140902800) （09140902800）
