实验动物与比较医学2012，Vol.32Issue(6)：499-502,4.DOI:10.3969/j.issn.1674-5817.2012.06.009
骨髓间充质干细胞对仓鼠皮肤深Ⅱ度烫伤的治疗作用及机制研究
Effect of Bone Marrow Mesenchymal Stem on Deep Partial Thickness Derma Burn Injury in Hamsters
摘要
Abstract
Objective To investigate the effect of bone marrow mesencymals stem cells transplantation on the deep partial thickness derma burn injury in hamsters and explore the possible mechanism. Methods Fourty hamsters were used to create deep partial thickness derma burn injury model and randomly divided into two groups: transplantation group (group A) and control group (group B). The wound of animals in group A were treated with local transplantation of the fifth generation cells of MSCs cultured in vitro with concentration of 1×l07 /ml plus surface daub of supernatant. The group B were treated with the same amount of saline. The wound healing effect was observed by HE staining, chymase mRNA level was analyzed on day 1, 3, 7, 14 after transplantation. Results The HE staining results showed the quality of healing was better in transplantation group than in control group. PCR results showed that chymase gene expression was found in both groups with the highest expression on day 3.Conclusion BMMSCs transplantations can promote wound healing, and dermal mastocyte chymotrysin would be involved in wound healing process.关键词
骨髓间充质干细胞/烫伤/仓鼠/糜蛋白酶Key words
Bone marrow mesenchymal stem cells/ Burn/ Hamster/ Chymase分类
生物科学引用本文复制引用
马嵋,张燕,魏琴,姜涛,段明军,张春,王雪梅,侯月梅..骨髓间充质干细胞对仓鼠皮肤深Ⅱ度烫伤的治疗作用及机制研究[J].实验动物与比较医学,2012,32(6):499-502,4.基金项目
新疆医科大学第一附属医院干细胞专项基金(2010GXB03) （2010GXB03）
