实验动物与比较医学2012，Vol.32Issue(6)：508-511,548,5.DOI:10.3969/j.issn.1674-5817.2012.06.011
两种耐甲氧西林金黄色葡萄球菌皮肤感染SKH-1无毛小鼠模型的比较研究
Comparative Research on Two Skin Infection Models in SKH-1 Hairless Mice Induced by MRSA
摘要
Abstract
Objective To explore methods to establish different models infected with methicillin resistant Staphylococcus aureus (MRSA). Methods Forty-eight specific pathogen free SKH-1 hairless mice were randomly divided into 4 groups. Mice from the PBS control groups were treated with sterilized PBS, by subcutaneous injection and direct daub on the puncture of skin injury by needle respectively, mice from model groups were treated with MRSA (ST-239) by subcutaneous injection or direct daub on the puncture of skin injury by needl. Models were evaluated by appearance and volume of abscesses or ulcers, CFU extracted from skin and morphology. Results There were significant differences in appearance, volume and CFU extracted from skin between the two models. Evident pathological alterations were observed. Skin of mice from subcutaneous injection group developed abscesses with limited inflammation, with no abscesses and diffuse inflammation induced in needle injury group. Conclusions Mice treated with MRSA by subcutaneous injection or diret daub on the puncture of skin injury by needl can develop abscesses model and wound-infected model respectively.关键词
耐甲氧西林金黄色葡萄球菌(MRSA)/皮肤感染/模型/SKH-1无毛小鼠Key words
Methicillin resistant Staphylococcus aureus(MRSA)/ Skin infection/ Model/ SKH-1 hairless mice分类
生物科学引用本文复制引用
陈雪雨,彭秀华,朱萍妹,徐春华,周文江,周光兴..两种耐甲氧西林金黄色葡萄球菌皮肤感染SKH-1无毛小鼠模型的比较研究[J].实验动物与比较医学,2012,32(6):508-511,548,5.基金项目
上海市科技发展基金项目(编号:09140900700) （编号:09140900700）
