同济大学学报（医学版）2012，Vol.33Issue(6)：10-14,5.DOI:10.3969/j.issn.1008-0392.2012.06.003
微小RNA-200a在肝癌中的表达及其功能
Expression of microRNA-200a in hepatic carcinoma cell line HepG2
郑莉莉 1李佳 1房林1
作者信息
- 1. 同济大学附属第十人民医院甲乳外科,上海200072
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-200a in hepatic cancer cell line HepG2 and its effect on biological features of hepatic carcinoma. Methods The expression of miR-200a was quantitatively examined by RT-PCR in HepG2 cells and normal liver QSG7701 cells. miR-200a mimics were transfected into HepG2 cells, the cell proliferation was assessed by MTT method, cell cycle and apoptosis were examined by flow cytometry and the migration of cells was assayed by Transwell test. Results The relative expression of miR-200a in HepG2 cells was 4. 79 times of that in QSG-7701 hepatic cells ( P < 0. 01 ). MTT assay revealed that 150 nmol/L miR-200a mimics significantly promoted proliferation of HepG2 cells at 72 h after transfection with a promotion rate of 38. 4%. Compared with controls, there was no significant changes in percentage of Go/Gl phase(57. 13 ±0.02)% , S phase cells(26.55 ±0.8)% and the proportion of early apoptotic cells(0.5 ±0.06)% of HepG2 cells after miR-200a mimics transfection(P >0.05). There were significant differences in cell migration between HepG2 cells after transfected with 100 nmol/L miR-200a and two control groups (0. 348 + 0.005 657 vs 0. 296 + 0. 006 364 and 0. 287 + 0. 005 364, P < 0. 05). Conclusion miR-200a expression in HepG2 cancer cells is up-regulated, which may be associated with hepatic cancer cell proliferation and invasion.关键词
肝肿瘤/微小RNA-200a/细胞增殖/侵袭Key words
liver cancer/ microRNA-200a/ proliferation/ invasion分类
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郑莉莉,李佳,房林..微小RNA-200a在肝癌中的表达及其功能[J].同济大学学报（医学版）,2012,33(6):10-14,5.
