生命科学研究2012，Vol.16Issue(6)：545-550,6.
G蛋白偶联受体在不同表达系统中高水平表达的研究进展
Advances in High-Level Expression of G Protein-Coupled Receptors in Different Expression Systems
摘要
Abstract
Guanosine-binding protein coupled receptors (GPCRs) belong to one of the largest super families of membrane proteins, with seven-transmembrane (7TM) domains. They mediate a wide range of physiological signals from the outside of the cell, which also are the targets for a large portion of pharmaceuticals drugs. The main researches of GPCRs focus on the structure, function and screening for drugs recently. Besides those, GPCRs also could be used as the response element for detecting of ligands. But it's difficulty extraction from organism because GPCRs with slowlybasal expression level in vivo. Consequently many researchers attempt to heterologous expression the GPCRs in vivo. The methods for high level expression GPCRs in different expression systems were reviewed. The aim is to provide some strategies for achieving high level and activated GPCRs from heterologous expression systems, laying foundations to probe the structure of GPCRs, relations between structure and function of GPCRs and to apply GPCRs in many research fields.关键词
G蛋白偶联受体(GPCRs)/体外表达系统/配基/表达水平Key words
guanosine-binding protein coupled receptors (GPCRs)/ expression system in vitro/ ligands/ expression level分类
生物科学引用本文复制引用
王迪,杨曙明,刘潇威..G蛋白偶联受体在不同表达系统中高水平表达的研究进展[J].生命科学研究,2012,16(6):545-550,6.基金项目
国家公益性行业(农业)科研专项经费项目(201203023) （农业）
