色谱2012，Vol.30Issue(12)：1271-1275,5.DOI:10.3724/SP.J.1123.2012.07024
高效液相色谱法考察不同炮制方法对知母中5种主要化学成分的影响
Effects of different processing methods on five main chemical constituents of Anemarrhena asphodeloides Bge.studied by high performance liquid chromatography
摘要
Abstract
A high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of neomangiferin, mangiferin, timosaponin B III, anemarrhe-nasaponin I and timosaponin A HI in the products of Anemarrhena asphodeloides Bge. processed by different methods. By comparing and analyzing the variation of the contents of the five components, the effects of processing on chemical constituents of Anemarrhena asphodeloides Bge. were explored, which provided evidences for the relevance between processing and the property changes of Anemarrhena asphodeloides Bge. The chromatographic separation was performed on an AlltimaTM C18 column (250 mm × 4. 6 mm, 5 μm) with a gradient elution of acetonitrile (A) and 0. 1% formic acid (B) (0 - 10 min, 5% A -20% A; 10 - 15 min, 20% A -25% A; 15 - 30 min, 25% A - 80% A; 30-35 min, 80% A - 100% A) at a flow rate of 0. 8 mL/min. Neomangiferin and mangiferin were detected by an ultraviolet detector at 265 nm and room temperature. Timosaponin BIII , anemarrhenasaponin I and timosaponin A III were detected by an evaporative light scattering detector with the drift temperature at 50 ℃ and gas pressure at 179. 1 kPa (26 psi). To some extent, the contents of the major components varied in different processed products of Anemarrhena asphodeloides Bge. The results indicated that different processing methods caused significant differences in the contents of the major components of Anemarrhena asphodeloides Bge. It is of great use for further researching the relevance of the processing methods to pharmacodynamics of Anemarrhena asphodeloides Bge.关键词
高效液相色谱/新芒果苷/芒果苷/知母皂苷BⅢ/知母皂苷Ⅰ/知母皂苷AⅢ/知母/炮制Key words
high performance liquid chromatography ( HPLC ) /neomangiferin/mangiferin/tiraosaponin B IH /anemarrhenasaponin I /timosaponin A m /Anemarrhena asphodeloides Bge. /processing分类
化学化工引用本文复制引用
赵路路,刘菲菲,彭缨,康廷国..高效液相色谱法考察不同炮制方法对知母中5种主要化学成分的影响[J].色谱,2012,30(12):1271-1275,5.基金项目
辽宁省博士启动基金项目(20111139)和辽宁省高等学校杰出青年学者成长计划资助项目(LJQ2011105). （20111139）
