山西大学学报（自然科学版）2012，Vol.35Issue(3)：520-524,5.
邻菲罗啉衍生物与人端粒G-四链体DNA的相互作用及细胞活性研究
Studies on the Interactions of Phenanthroline Derivatives with Human Telomere G-quadruplex DNA and on Their Effects on the Cell Activity
摘要
Abstract
The interactions between three Phenanthroline derivatives and the human telomeric G-quadruplex DNA were studied by absorption titration, fluorescence titration and PCR stop assay in PBS solution containing 100 mmol · L-1 KCl. In addition,MTT assay was used to study the effect of the compounds on the proliferative activity of human HepG2 cells. The results showed that the compounds could bind G-quadruplex with affinity constant of 1×106 mol-1 · L,and stabilized significantly the structure of G-quadruplex. At the same time, the compounds could inhibit the proliferation of HepG2 cells in micromolar concentration.关键词
邻菲罗啉/端粒/G-四链体/细胞增殖Key words
Phenanthroline/ telomere/ G-quadruplex/ cell proliferation分类
化学化工引用本文复制引用
王立华,文烨,刘洁,张芳芳,魏春英..邻菲罗啉衍生物与人端粒G-四链体DNA的相互作用及细胞活性研究[J].山西大学学报（自然科学版）,2012,35(3):520-524,5.基金项目
国家自然科学基金(21171108) （21171108）
教育部博士点专项基金(20101401110009) （20101401110009）
山西省自然科学基金(2009011012-2) （2009011012-2）
太原市大学生创新创业项目(20103007) （20103007）
