实用医学杂志2012，Vol.28Issue(11)：1753-1755,3.DOI:10.3969/j.issn.1006-5725.2012.11.001
敲减Akt表达对类风湿关节炎成纤维样滑膜细胞增殖的影响
Effects of down-regulating of Akt protein on the proliferation of rheumatoid arthritis fibroblast-like synoviocytes
摘要
Abstract
Objective To study the effects of lentiviral vector-mediated small interfering RNA targeting Akt protein on the proliferation of rheumatoid arthtitis (RA) fibroblast-like synoviocytes (FLS). Methods Small interfering RNA targeting Akt protein in RA FLS was expressed, and then the expression of Akt was detected by RT-PCR and Western Blot, and the proliferation of RA FLS was measured by MTT method. Results The expressions of Aktl and Akt2 mRNA were decreased by more than 65% and 68% respectively in RA FLS after being transfected with lentiviral vector pL/Akt. The expression of Akt protein was reduced by 76.19% 48 h later (P ＜ 0.05), and the proliferation of RA FLS was decreased by 27.50%, 44.69%, and 56.80% (P ＜ 0.05) at 48 h, 72 h, and 96 h later. Conclusion Lentiviral vector pL/Akt could decrease the expression of Akt protein, and significantly inhibit RA FLS proliferation.关键词
关节炎,类风湿/Akt/RNA干扰/慢病毒表达载体Key words
Arthritis, rheumatoid/ Akt/ RNA interfering/ Lentivirus vector引用本文复制引用
徐宁,王栋,庄俊华,李曼,黎小琼,黄宪章..敲减Akt表达对类风湿关节炎成纤维样滑膜细胞增殖的影响[J].实用医学杂志,2012,28(11):1753-1755,3.基金项目
广东省自然科学基金项目(编号:10151040701000031) （编号:10151040701000031）
广东省中医院拔尖人才基金项目(编号:2011-84) （编号:2011-84）
