实用医学杂志2012，Vol.28Issue(11)：1756-1758,3.DOI:10.3969/j.issn.1006-5725.2012.11.002
吲哚美辛对结肠癌细胞HCT116的影响及机制探讨
Effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 and its mechanisms
摘要
Abstract
Objective To study the effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 and its mechanisms. Methods The effects of indomethacin on the proliferation and invasion of colon cancer cell line HCT116 were measured by WST-8 method and TransweU chamber in vitro. The expressions of p-catenin, cyclin Dl, and matrix metalloproteinase-7 (MMP-7) protein were detected by western blotting method after indomethacin treatment. Results The proliferation of HCT116 was inhibited by indomethacin in a time and dose dependent manner, and the IC50 of 24 h, 48 h, and 72 h were 440.36 μmol/L, 323.9 μxmol/L, and 254.37 μmol/L, respectively; and the invasion of HCT116 was also inhibited by indomethacin in a dose dependent manner. The expressions of fi-catenin, cyclin Dl, and MMP7 were reduced after indomethacin treatment. Conclusions Indomethacin can inhibit the proliferation and invasion of colon cancer cell line HCT116, which may through the Wilt /(3-catenin signal pathway.关键词
结肠肿瘤吲哚美辛/β-连环素/细胞周期素D1/基质金属蛋白酶7Key words
Indomethacin/ β-catenin/ Cyclin Dl/ Matrix metalloproteinase7引用本文复制引用
王国强,方洧靖,卢震海,潘志忠,万德森..吲哚美辛对结肠癌细胞HCT116的影响及机制探讨[J].实用医学杂志,2012,28(11):1756-1758,3.基金项目
广东省科技计划项目(编号:2008B080701025) （编号:2008B080701025）
