实用医学杂志2012，Vol.28Issue(16)：2678-2681,4.DOI:10.3969/j.issn.1006-5725.2012.16.012
Jagged1RNA干扰慢病毒载体抑制舌癌Cal-27细胞增殖、侵袭作用的研究
Lentiviral vector-mediated RNA interference of Jagged1 expression suppresses the proliferation and invasion of tongue carcinoma Cal-27 cells
摘要
Abstract
Objective To investigate the role of lentivirus-mediated Jaggedl RNA interference in inhibiting the proliferation and invasion of tongue cancer cell Cal-27. Methods RT-PCR and Western blot assay was used to determine the knock-down efficiency of Jaggedl by RNA interference in Cal-27 cells. Clone formation experiment was performed to detect cell colony dependence and reproductive activity. Cell cycle was determined by flow cytometry, and cell invasion activity was determined by the chamber cell invasion assay. Results The siRNA of KD group had significant knock-down effect on Jaggedl. Colony formation of Cal-27 cells was significantly reduced in the KD group. The percentage of S-phase in Cal-27 cells was significantly reduced in the KD group after transfection with the lentiviral vectors. The percentage of S-phase in Cal-27 cells in the KD group decreased by 62.98% compared to that in the NC group. The percentage of Gl-phase in Cal-27 cells in the KD group increased. Apoptosis rate of Cal-27 cells was significantly increased in the KD group. The cell growth in the KD group was inhibited at the rate of 32.50%. The cell invasion rate was not changed obviously after transfection with Jaggedl-targeted RNAi lentiviral vector. Conclusion Targeting Jaggedl RNA interference can efficiently decrease Jaggedl mRNA and protein expressionin Cal-27 cells, resulting in inhibition of tongue cancer cell proliferation s. Jaggedl is a promising new target for the treatment of tongue cancer.关键词
舌肿瘤/Notch/Jagged1/RNA干扰/慢病毒载体Key words
Tongue neoplasms/ Notch/ Jagged1/ RNA interference/ Lentiviral vector引用本文复制引用
张同韩,刘海潮,梁玉洁,梁立中,郑广森,廖贵清,吴纪楠,黄洪章..Jagged1RNA干扰慢病毒载体抑制舌癌Cal-27细胞增殖、侵袭作用的研究[J].实用医学杂志,2012,28(16):2678-2681,4.基金项目
国家自然科学基金(编号:30772442) （编号:30772442）
第5批中国博士后科学基金面上项目(编号:2012M511877) （编号:2012M511877）
