实用医学杂志2012，Vol.28Issue(17)：2867-2869,3.DOI:10.3969/j.issn.1006-5725.2012.17.017
结核分枝杆菌荧光定量聚合酶链反应对结核性脑膜炎的早期诊断价值
The Early Diagnostic Value of Mycobacterium Tuberculosis Fluorescence Quantitative PCR in Tuberculous Meningitis
吴睿彦 1陈志成 1肖芃
1邱薇 1陈品儒 1王维勇 1岑文昌 1陈慧玉1
作者信息
- 1. 510095,广州市胸科医院重症结核科1内科
- 折叠
摘要
Abstract
Objective The objective of this paper is to discuss the application value of fluorescence quantitative PCR in detection of tuberculous meningitis. Methods 203 tuberculous meningitis patients were operated lumbar puncture. The samples were detected by mycobacterium tuberculosis fluorescence quantitative PCR, mycobacterium tuberculosis culture and smear microscopy to find acid-fast bacilli. Then detection sensitivities and specificities of these methods were compared. Results The positive rate in fluorescence quantitative PCR to find acid-fast bacilli was 52.7%, much higher than 1.2% in cerebrospinal fluid smear microscopy and 11.5% in mycobacterium tuberculosis culture. Comparing fluorescence quantitative PCR with the method of mycobacterium tuberculosis culture, P＜ 0.01, differences has statistically significant, which shows fluorescence quantitative PCR is better than mycobacterium tuberculosis culture. Conclusion Fluorescence quantitative PCR of mycobacterium tuberculosis in the diagnosis of tuberculous meningitis is with a high specificity and a short annalistic period. It is of important reference value in the early diagnosis of tuberculous meningitis.关键词
结核,脑膜/结核分枝杆菌/聚合酶链反应Key words
tuberculous meningitis/ polymerase chain reaction (PCR)引用本文复制引用
吴睿彦,陈志成,肖芃
,邱薇,陈品儒,王维勇,岑文昌,陈慧玉..结核分枝杆菌荧光定量聚合酶链反应对结核性脑膜炎的早期诊断价值[J].实用医学杂志,2012,28(17):2867-2869,3.
