实用医学杂志2012，Vol.28Issue(18)：3012-3015,4.DOI:10.3969/j.issn.1006-5725.2012.18.012
HCV多表位复合抗原基因表达条件的探索
Establishment of conditions for HCV multi-epitope antigen gene expression
摘要
Abstract
Objective To screen E.coli strain for HCV multi-epitope antigen gene (PCX) expression, and to optimize conditions for PCX expression and to detect the immune specificity of the expressed product. Methods The recombinant plasmid, pWR450-PCR, containing HCV multi-epitope antigen gene was transformed into DH5a, TOP10F' and BL21 strains,respectively, and the fusion protein (GZ-PCX) was induced to express by different concentrations of IPTG for different periods and in different medium. Then the immune specificity of the expressed protein was identified by Western blotting assay with the HCV positive serum. Results The recombinant plasmid, which was transformed into DH5a, TOP10F' strains, could express the fusion protein with immune specificity, but the recombinant plasmid transformed into BL21 strains failed to express corresponding product. The target protein expression in the TB medium was significantly higher than that in the LB medium. Conclusion The target protein can be expressed efficiently at 3 hours after 1 mM IPTG induction at 37^. The antigen prepared has a certain immune specificity and immunogenicity.关键词
丙型肝炎病毒/原核表达/免疫特异性/免疫原性Key words
Hepatitis C virus/ Prokaryotic expression/ Immune specificity/ Immunogenicity引用本文复制引用
杨蓉,杨婷,李华,龙润乡,董承红,董丽娟,谢忠平..HCV多表位复合抗原基因表达条件的探索[J].实用医学杂志,2012,28(18):3012-3015,4.基金项目
国家863项目(编号:2007AA02Z480) （编号:2007AA02Z480）
云南省联合支持国家计划项目(编号:2008GA008) （编号:2008GA008）
