实用医学杂志2012，Vol.28Issue(19)：3170-3173,4.DOI:10.3969/j.issn.1006-5725.2012.19.005
拟Smac合成肽羧甲基壳聚糖磁性纳米复合物的制备及体外抗肿瘤性能研究
The preparation and antineoplastic activity in vitro of Smac-mimic peptide-loaded O-carboxylmethylated chitosan magnetic nanoparticles
摘要
Abstract
Objective To prepare Smac-mimic peptide-loaded O-carboxylmethylated chitosan (O-CMC) magnetic nanoparticles (SmacN7-0-CMC-MNPs) ,and to investigate its effects on the apoptosis of bladder carcinoma T24 cells in vitro when combined with constant external magnetic fields. Methods The magnetic SmacN7-0-CMC-MNPS was composed by O-CMC, SmacN7, and Fe3O4 nanoparticles. And its physicochemical characteristics were confirmed by TEM and vibrating sample magnetometer. The apoptosis of bladder carcinoma T24 cells was observed by Hoechst33258 staining and the inhibition rates (IR) was assayed by MTT when SmacN7-0-CMC-MNPs was combined with constant external magnetic fields. Results The mean diameter of SmacN7-0-CMC-MNPs was 46.2 nm with round shape and the loading dose and trapping efficiency was (31.8?.6)% and (65.2?.4)%, respectively. Magnetization curve showed superparamagnetism. SmacN7-0-CMC-MNPs nanoparticles could apparently induce apoptotic appearance and inhibit the growth of bladder cancer cells when combined with constant external magnetic fields. Conclusion SmacN7-0-CMC-MNPs had small particle size, good magnetic responsiveness, high loading rate and embedding ratio. It can sharply promote apoptosis of bladder cancer cells when combined with constant external magnetic fields and implies a promising future for the biological therapy for bladder cancer.关键词
纳米粒子/Smac,合成肽/O-羧甲基壳聚糖/凋亡Key words
Nanoparticles/ Smac, synthetic peptide/ O-carboxylmethylated chitosan/ Apoptosis引用本文复制引用
王竞,曾甫清,汪良,蒋国松,黄慧,汪涛,潘晖..拟Smac合成肽羧甲基壳聚糖磁性纳米复合物的制备及体外抗肿瘤性能研究[J].实用医学杂志,2012,28(19):3170-3173,4.基金项目
教育部博士学科点专项科研基金课题(编号:20060487066) （编号:20060487066）
