实用医学杂志2012，Vol.28Issue(19)：3288-3291,4.DOI:10.3969/j.issn.1006-5725.2012.19.050
西尼罗河病毒实时荧光定量PCR检测方法的建立及其应用研究
Development and application of a real-time fluorescence quantitative PCR method for detecting West Nile virus
摘要
Abstract
Objective To develop a real-time fluorescence quantitative PCR method for detecting West Nile virus. Methods Primers were designed according to the conserved sequence of the envelope protein (E) of West Nile virus. SYBR Green-based real-time PCR was established. The specificity, sensitivity and stability of the method were assessed. 54 clinical specimens were detected by the established SYBR Green-based real-time PCR. Results High specificity was found using this established method to detect West Nile virus and no cross reactions were observed for other viruses such as JEV and SLEV. The sensitivity of this method was low to 100 pg. The correlation coefficient and slope value of standard curve were 0.995 and -3.444, respectively. The accuracy of clinical diagnosis was 98.15% for 54 clinical specimens. Conclusion Real time quantitative PCR was a reliable, specific, sensitive and useful tool for detecting West Nile virus.关键词
西尼罗河病毒/实时荧光定量PCR/特异性/临床诊断/日本脑炎病毒Key words
Objective To develop a real-time fluorescence quantitative PCR method for detecting West Nile virus. Methods Primers were designed according to the conserved sequence of the envelope protein (E) of West Nile virus. SYBR Green-based real-time PCR was estab引用本文复制引用
李林海,陈丽丹,廖杨,陈建芸,石玉玲..西尼罗河病毒实时荧光定量PCR检测方法的建立及其应用研究[J].实用医学杂志,2012,28(19):3288-3291,4.基金项目
广东省科技计划项目(编号:2010B031000024) （编号:2010B031000024）
