实用医学杂志2012，Vol.28Issue(20)：3457-3459,3.DOI:10.3969/j.issn.1006-5725.2012.20.056
结核分枝杆菌氟喹诺酮耐药与gyrA基因突变的关系
Characterization of gyrA gene mutation in fluoroquinolones-resistant Mycobacterium tuberculosis
摘要
Abstract
Objective To investigate the characterization of gyrA gene mutation in fluoroquinolones-resistant Mycobacterium tuberculosis. Methods 60 Mycobacterium tuberculosis clinical strains, including 30 drug-resistance strains and 30 drug-susceptible strains, were identified and their drug-resistance in phenotype were tested by BACTEC-MGIT960, gyrA gene was amplified by PCR and then fragments were cloned into T-vector and sequenced. Results Among the 30 Mycobacteria tuberculosis drug-susceptible isolates, no sense mutation was identified in QRDR.19 mutant strains at 90,91 or 94 site of gyrA gene were identified in 30 drug-susceptible strains. There were 63.3% strains with gyrA mutation among 30 drug-resistant isolates in phenotype. Conclusion There is closely association between gyrA gene mutation and fluoroqunolones-resistant in phenotype for Mycobacterium tuberculosis.关键词
结核分枝杆菌/氟喹诺酮类/耐药/gyrA/基因突变Key words
Mycobacterium tuberculosis/ Fluoroquinolones/ Resistance/ GyrA/ Gene mutations引用本文复制引用
杨辉,张国良,张明霞,吴爱武,陈建波,陈心春..结核分枝杆菌氟喹诺酮耐药与gyrA基因突变的关系[J].实用医学杂志,2012,28(20):3457-3459,3.基金项目
国家自然科学基金资助项目(编号:81172732) （编号:81172732）
