天津中医药2012，Vol.29Issue(6)：583-587,5.
胡黄连中酚类化合物的分离及UPLC-ESI-MS分析
Isolation of phenolic constituents from Picrorhiza scrophulariiflora Pennell and UPLC-ESI-MS analysis
摘要
Abstract
[Objective] To study the Phenolic constituents in Picrorhiza scrophulariiflora Pennell and determine their contents in the medicinal materials. [Methods] The compounds were isolated by the solvent extract, silica gel and Sephadex LH-20 columns and their structure were elucidated by 1H-NMR、13C-NMR and UPLC-ESI-MS which were used as analysis standards. Their contents in the extract and medicinal materials were determined by UPLC-ESI-MS. [Results] Six phenolic compounds were obtained and identified as Picein (Ⅰ), Androsin (Ⅱ), Scrophenoside A(Ⅲ), Scrophenoside F(Ⅳ), Vanillic acid (Ⅴ) and Acetovanillone(Ⅵ). [Conclusion] The contents of above compounds in the extract are 1.50%, 5.62%, 3.53%, 1.96%, 0.78% and 3.16%, and the contents in the medicinal materials are 0.24%, 0.90%, 0.56%, 0.31%, 0.13%, 0.51%, respectively.关键词
胡黄连/UPLC-ESI-MS/酚类化合物/分离Key words
Picrorhiza scrophulariiflora Pennell/ UPLC-ESI-MS/ Phenolic constituents/ isolation分类
医药卫生引用本文复制引用
刘时乔,张新鑫,单淇,周福军,侯文彬..胡黄连中酚类化合物的分离及UPLC-ESI-MS分析[J].天津中医药,2012,29(6):583-587,5.基金项目
天津市中药对照品库的建设项目(033187511). （033187511）
