现代妇产科进展2012，Vol.21Issue(11)：857-860,4.
外源性三磷酸腺苷对高表达P2X7受体的宫颈癌Caski细胞生长的影响
Effect of ATP on proliferation of cervical cancer Caski cells with P2X7 receptor over-experssion
摘要
Abstract
Objective: To investigate the effects of extracellular adenosine-5 '-triphos-phate( ATP) on proliferation and apoptosis of cervical cancer Caski cells with P2X7 receptor o-ver-experssion. Methods: The Caski-P2X7 cells and Caski-3. 1 control cells (transfected mock plasmids) with different concentrations of ATP which were transfected and identified in my laboratory ago. MTT assay was used to assess the viability of cells. The change in cell membrane potential was measured by fluorescence staining. The apoptotic rates of these cells were detected by flow cytometry. After treatment with 2. 5mmol/L ATP,the levels of apoptosis-related proteins (Cytochrome C, bcl-2, Bax) of two cells were tested by Western blot. Results: Two kinds of cell survival rate and mitochondrialmembrane potential were decreased, apoptosis rate increased, due to the treatment of different ATP concentration, and showed a dose-dependent. Caski-P2X7 cells survival rate and mitochondrialmembrane potential were significantly lower than that in Caski-3. 1 cells under ATP treatment (P<0. 05) ,but apoptosis rate was dramatically higher than that in Caski-3. 1 cells(P<0. 05). After treatment with 2. 5mmol/L ATP,the expressions of cytochrome C and apoptosis protein Bax were increased significantly in Caski-P2X7 cell cytoplasm, and the expression of anti-apoptotic protein bcl-2 was significantly decreased. Conclusion: A certain concentration of ATP could induce apoptosis of Caski cells through P2X7 receptor pathway.关键词
子宫颈肿瘤/Caski细胞/嘌呤能P2X7受体/三磷酸腺苷/细胞凋亡Key words
Uterine cervical neoplasms/Caski cell/Purinergic P2X7 receptors/Adenosine triphosphate/Apoptosis分类
医药卫生引用本文复制引用
孙丽,韩莉,石慧,谭超,神谢静,刘朝奇..外源性三磷酸腺苷对高表达P2X7受体的宫颈癌Caski细胞生长的影响[J].现代妇产科进展,2012,21(11):857-860,4.基金项目
湖北省自然科学基金资助项目(No:2009CDZ024) （No:2009CDZ024）
