现代妇产科进展2012，Vol.21Issue(11)：861-864,4.
AMPK/ERK信号通路在脂联素抑制子宫内膜癌细胞增殖中的作用
Inhibitory effect on proliferation induced by adiponectin in endometrial cancer cells via AMPK/ERK signaling pathway
摘要
Abstract
Objective:To explore inhibitory effect of adiponectin on endometrial cancer cell proliferation and AMPK/ERK signal transduction mechanism. Methods; After pretreatment with 10μg/ml adiponectin for 0 ~60min,the phosphorylation of AMPK and ERK in endometrial cancer Ishikawa3-H-12 cell were detected by Western blot. The levels of Cyclin Dl mRNA and protein induced by 12h and 24h treatment with adiponectin were detected by RT-PCR and Western blot respectively. After 48h adiponectin treatment, proliferation of endometrial carcinoma cells was observed by methyl thiazolyl tetrazolium (MTT) assay. Results: Adiponectin induced AMPK phosphorylation of endometrial cancer cell in time-dependent manner. Activation of AMPK ocuured at 5min and lasted at least 30min (F=22.749 ,P = 0.000). The AMPK activation induced by Adiponectin was blocked by AMPK inhibitor comfound C. Adiponectin inhibited ERK phosphorylation in time-dependent manner. ERK activation decreased at 5min and continued to 60min at least (F= 13. 802 ,P = 0. 000). Comfound C abrogated ERK inhibition of adiponectin. Both Cyclin Dl mRNA and protein expressions were inhibited by adiponectin (P = 0.003,P-0.000 respectively). These efftects were blocked by comfound C (P = 0.006,P = 0.000 respectively). After adiponectin treatment, proliferation of endometrial cancer cells was lower than that of control group (P = 0. 001 ). Comfound C abrogated the inhibitory effect induced by adiponectin on the proliferation of endometrial cancer cells ( P = 0. 002 ). Conclusion : Adiponectin maybe inhibit proliferation of endometrial cancer cells through AMPK/ERK/ Cyclin Dl signal pathway.关键词
子宫内膜癌/脂联素/AMPK/ERK/细胞周期素D1/增殖Key words
Endometrial carcinoma/Adiponectin/AMPK/ERK/Cyclin Dl/Proliferation分类
医药卫生引用本文复制引用
张丽志,温克,苏健,瞿全新..AMPK/ERK信号通路在脂联素抑制子宫内膜癌细胞增殖中的作用[J].现代妇产科进展,2012,21(11):861-864,4.基金项目
天津市卫生局科技基金重点项目(No:2011KR04) （No:2011KR04）
