现代妇产科进展2012，Vol.21Issue(11)：878-881,4.
模拟子宫内膜微环境体外诱导兔骨髓间充质干细胞向子宫内膜上皮细胞分化的实验研究
Differentiation of rabbits bone marrow mesenchymal stem cells in to endometrial epithelial cells in simulate endometrial microenvironment in vitro
摘要
Abstract
Objective: To investigate the feasibility of differentiation of rabbits bone mesenchymal stem cells ( BMSCs) in to endometrial epithelial cells in simulate endometrial microenvironment in vitro. Methods: Rabbits BMSCs were isolated and identified, and endometrial conditioned medium was extracted. BMSCs were induced by endometrial conditioned medium and estrogen ( p-estradiol 1×l0-7mol/L) in vitro. Immunofluorescence and Western blot were used to test the expression of epithelial marker. Results: Rabbits BMSCs in vitro showed great proliferative potential and clonal growth. The positive rates of CD44 and CD90 in BMSCs were 99.91% and 98.64% respectively,and the expression of CD45 was negative. BMSCs morphology was differentiated from stromal cells to epithelioid cells by cultured in the endometrial conditioned medium and estrogen for 5 days. The epithelial marker cytokeratin was positive in immunofluorescence analysis in the differentiated BMSCs, and the expression levels of cytokeratin in the experimental group were higher than those in the control group through Western blot (P< 0. 05). Conclusion:Bone marrow mesenchymal stem cells have the potential to differentiate in to endometrial epithelial cells induced by endometrial conditioned medium and estrogen. The simulate endometrial microenvironment plays an important role in the process of MSCs differentiation.关键词
骨髓间充质干细胞/宫腔粘连/微环境/分化Key words
Bone marrow mesenchymal stem cells/intrauterine adhesions/microenvironment /differentiation分类
医药卫生引用本文复制引用
刘芳,何援利..模拟子宫内膜微环境体外诱导兔骨髓间充质干细胞向子宫内膜上皮细胞分化的实验研究[J].现代妇产科进展,2012,21(11):878-881,4.基金项目
国家自然基金资助项目(No:81270658) （No:81270658）
