现代妇产科进展2012，Vol.21Issue(12)：941-944,4.
出生早期使用二甲双胍对宫内高雄激素化仔鼠成年后卵巢CYP17的影响
Expression of CYP17 in ovarian tissue of adult rat model with hyperandrogenism in uterus and treatment by metformin in newborn
摘要
Abstract
Objective:To construct polycystic ovary syndrome(PCOS) rat model by mater-nal exposure to excess testosterone,examine the CYP17 and metabolism in the rat models after trea-ted by metformin in newborn. Methods: Wistar female rats were randomly divided into model group and control group. On days 16～18 of pregnancy, wistar female rats were injected S. C. with testoster-one propionas 2. 5 mg or sesame oil daily for 3 consecutive days. Pregnant rats after delivery 2 days were gavaged by metformin 450mg/(kg·d) and saline for 14 consecutive days. Adult female off-springs were studied. Serum concentrations of sex hormones luteinizing hormone,Follicle-stimulating hormone,Testosterone,insulin were measured. CYP17 protein expression in ovary were determined by immunohistochemistry and Western blot. Result: ( 1) The female rats that were prenatally androg-enized had polycystic ovary. Serum levels of testosterone, luteinizing hormone were significantly higher. The insulin levels and Follicle-stimulating hormone had not changed. Western blot showed that the expression of CYP17 in model ovarian tissues were higher. (2) The prenatally androgenized female rats that were treated by metformin in newborn had normal ovary. Serum hormone levels and the expression of CYP17 returned to normal. Conclusion: Prenatally androgenized female rats exhibi-ted hyperandrogenism,high luteinizing hormone and polycystic ovary,which is coincident with the characteristics of human PCOS. Metformin by adjusting the serum hormone levels and ovarian CYP17 expression played a role in improve the clinical symptoms of prenatal androgen mice.关键词
多囊卵巢综合征/丙酸睾酮/二甲双胍/类固醇17-α-羟化酶/基因,CYP17/Wistar雌性大鼠Key words
Polycystic ovary syndrome/Testosterone propionate/Metformin/Steroid 17-alpha-hydroxylase/Genes,CYP17/Wistar female rats分类
医药卫生引用本文复制引用
樊佳丽,段涛,周倩..出生早期使用二甲双胍对宫内高雄激素化仔鼠成年后卵巢CYP17的影响[J].现代妇产科进展,2012,21(12):941-944,4.基金项目
上海市卫生系统新百人计划资助(No:XBR2011064) （No:XBR2011064）
上海市科委实验动物专项基金资助(No:11140901900) （No:11140901900）
教育部高等学校博士点基金(No:2011007210005) （No:2011007210005）
