厦门大学学报（自然科学版）2012，Vol.51Issue(6)：1085-1091,7.
海绵共附生放线菌HNS054菌株的鉴定及发酵液抑菌活性研究
Identification and Bioactivity Screening of Streptomyces sp. HNS054 Associated with Marine Sponge Mycale sp.
摘要
Abstract
Sponge-associated bacteria are the potential sources of nature bioactive products. Marine sponge Mycale sp. is distributed throughout the intertidal zone of Fujian sea area which is located in the southeastern part of China. Large numbers of bacteria including Actinobacteria group were isolated from Mycale sp. and test for antimicrobial activities was performed in previous study. Among all isolates,the strain HNS054 showed strong activities against Gram-positive bacteria. Multilocus sequence typing (MLST) by using the 16S rRNA,atpD, recA,rpoB and trpB genes identified strain HNS054 as Streptomyces labedae. Phenotypic observation and physiological characteristics further confirmed that HNS054 belong to S. labedae. Comparison with commercial antibiotics (Ampicil-lin,Streptomycin, CPFX, Vancomycin and Achromycin) , strain HNS054 exhibited more obvious antibacterial activities. Analysis of the crude extract of strain HNS054 including temperature-sensitive, tolerating high pH and exhibiting strong light absorption near 230 nm could provide data for further study.关键词
山海绵/放线菌/抑菌活性/鉴定Key words
Mycale sp. jactinomycete/antimicrobial activity/ identification分类
生物科学引用本文复制引用
苏培,王德祥,柯才焕,黄妙琴,赵晶..海绵共附生放线菌HNS054菌株的鉴定及发酵液抑菌活性研究[J].厦门大学学报（自然科学版）,2012,51(6):1085-1091,7.基金项目
中央高校基本科研业务费专项(2010121034) （2010121034）
福建省自然科学基金项目(2011J01245) （2011J01245）
