畜牧兽医学报2012，Vol.43Issue(12)：1949-1956,8.
鸭CD8α基因克隆及不同禽类分子进化分析
Molecular Cloning and Evolution of CD8α Gene in Duck Populations
摘要
Abstract
The coding sequence of CD8aα was cloned by RT-PCR and LD-PCR in five duck populations (Jinding Duck, Cherry Valley duck, muscovy, mallard and spot-billed duck). The 9 sequences (another 4 sequences from GenBank) were analyzed by online software of bioinformatics (the best nucleotide substitution model) was selected by Jmodeltest, the nucleotide substitution saturation was assessed with DAMBE, and the selective pressure on amino acid sites was tested by PAML. The results of sequencing showed the coding sequence of CD8α was conservative except a few point mutations. The model "GTR + G" was selected as the best nucleotide substitution model, the sequences were experienced full substitution unsaturation. The phylogenetic trees of CD8α among different fowl were generated by MEGA 5. 0, and the 9 fowl were divided into 3 parts according to CD8α sequences. The 7 amino acid sites undergoing positive selection were detected, 4 of them were in the important function regions by the site-specific model. In summary, the coding sequence of CD8α was cloned successfully,and it was conservative in molecular evolution, which may be helpful for studying evolution, structure and function of CD8α among fowl.关键词
鸭/CD8α/克隆/进化Key words
duck/ CD8a/ molecular cloning/ evolution分类
农业科技引用本文复制引用
徐琪,黄正洋,孙志明,陈阳,赵文明,张扬,李秀,段修军,陈国宏..鸭CD8α基因克隆及不同禽类分子进化分析[J].畜牧兽医学报,2012,43(12):1949-1956,8.基金项目
国家自然科学基金项目(31101704) （31101704）
现代农业产业技术体系建设专项资金(CARS-43-3) （CARS-43-3）
江苏高校优势学科建设工程资助项目(苏政办发[2011]137号) （苏政办发[2011]137号）
