畜牧兽医学报2012，Vol.43Issue(12)：1984-1990,7.
出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达
Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development
摘要
Abstract
The objective of the experiment was to study the localition and the average integrated optical density of ERa and PR immunopositive cells and the abundance of ERa mRNA and PR mRNA in mammary gland during postnatal development in Jining Gray goat. The SP immunohis-tochemical staining and Image analysis methods were used to localize ERa and PR immunoprotec-tive(-ip) cells in mammary gland of Jining Gray goat. The relative expression of ERa mRNA and PR mRNA was analyzed by using the real time fouorescence quantitative PCR with GAPDH as an internal gene. ERa and PR had similar variations, immunoprotective staining was mainly localized in the nucleus of blood vessels endothelial cells, stromal cells and ductal epithelial cells of mammary gland, occasionally in cytoplasmic, PR-ip substance also found in alveolar epithelial cells in 180 days. The average optical density was gradually increased from birth day to sexual maturity, and it was significantly higher in sexual maturity than that in birth day and puberty (P<C 0.01) ,it was not different with that in 180 days (P>0. 05). The results of qRT-PCR showed that the abundance of ERa mRNA was gradually increased from birth day to sexual maturity , and it was significantly higher in sexual maturity than that in birth day and puberty (P<0. 01) , there was a slightly decrease from sexual maturity to 180 days . While the abundance of PR mR-NA from birth day to sexual maturity was lower and there was no significant difference during these groups. In 180 days, it was significantly higher than the other three groups (P<0. 01). These results indicated that the estrogen receptors of alph and the progesterone receptors could promote the growth of glandular ,the hyperplasia of epithelial cells and the development of lobu-lo-alveolar during postnatal development in Jining Gray goat.关键词
济宁青山羊/乳腺/雌激素受体α/孕激素受体/免疫组织化学/qRT-PCRKey words
Jining Gray goat/ mammary gland/ ERa/ PR/ immunohistochemistry/ qRT-PCR分类
农业科技引用本文复制引用
尹玉涛,王树迎,刘宵,张德敏,侯衍猛,黄丽波..出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达[J].畜牧兽医学报,2012,43(12):1984-1990,7.基金项目
国家自然科学基金项目(30871832) （30871832）
山东省自然科学基金(ZR2009DM027) （ZR2009DM027）
山东省科技攻关项目(2008GG30008018) （2008GG30008018）
