西南国防医药2012，Vol.22Issue(12)：1277-1280,4.DOI:10.3969/j.issn.1004-0188.2012.12.001
靶向HBV C基因的siRNA在HepG2.2.15细胞中对HBV的抑制作用
Inhibitory effect of targeting C gene siRNA on hepatitis B virus in HepG 2.2.15 cells
摘要
Abstract
Objective To study the inhibitory effect of shRNA expression plasmid of HBV C gene on HBV in HepG 2. 2. 15 cells. Methods Two shRNA expression plasmids (S1 and S2) of targeting HBV C gene and one nonhomologous shRNA expression plasmid (S3) used for the control were designed and constructed. After the transfection of HepG 2.2.15 cell, the HBV C gene expressionand expressions of HBV, DNA, HBsAg, and HBeAg in the supernatant were respectively detected by Real Time PCR and EI2SA methods. Results After the transfection for 24 h, the mRNA expression of HBV C gene significantly decreased by 58% - 83% ; the expression of HBV DNA decreased by 2.44 - 3.36 log values; the expression levels of HBsAg and HBeAg decreased by 51% - 79% and 50% - 77% , respectively. Combined application of SI and S2 could enhance the inhibitory effect on HBV, while S3 had no such effect. Conclusion The shRNA expression plasmids SI and S2 and the combined application of both two can effectively inhibit the replication and expression of HBV in HepG 2.2.15 cells.关键词
乙型肝炎病毒/RNA干扰/小干扰RNA/短发夹状RNAKey words
hepatitis B virus/ RNA interference/ small interfering RNA/ short hairpin RNA分类
医药卫生引用本文复制引用
肖安,刘斌,焦晔,赵欣,刘明秋,严维耀,郑兆鑫..靶向HBV C基因的siRNA在HepG2.2.15细胞中对HBV的抑制作用[J].西南国防医药,2012,22(12):1277-1280,4.基金项目
国家自然科学基金(30972629) （30972629）
