疑难病杂志2012，Vol.11Issue(12)：926-928,3.DOI:10.3969 / j.issn.1671-6450.2012.12.011
外源性转化生长因子-β1作用下胎儿软骨终板细胞II型胶原与蛋白多糖的相关性
The correlation between collagen II and proteoglycan of the fetal endplate cartilage cells cultured by the transforming growth factor-β1 in vitro
李全修 1蔡月艳 2刘欣 1樊培新 1刘勇3
作者信息
- 1. 518010,广东省深圳平乐骨伤科医院急诊骨科
- 2. 274500,山东省东明县中医院影像科
- 3. 266003,青岛大学医学院附属医院脊柱外科
- 折叠
摘要
Abstract
Objective To observe the correlation and the change of collagen II and proteoglycan of the fetal endplate cartilage cells cultured by the transforming growth factor-p, ( TGF-β1 ) in vitro. Methods The second generation fetal endplate cartilage cells for 0, 1, 2, 3, 4, 5 or 6 days with TGF-β11( 10μg/L ) in vitro. The proteoglycan, collagen II gene and protein expression were detected by Western blot or RT-PCR. Results There was not significant difference of the expression of collagen Hand proteoglycan mRNA before 1d and after 4d( P >0. 05 ), however, the expression of collagen II and proteoglycan mRNA increased gradually with a significant difference from 1d to 4d( P<0.05), and collagen II change with proteoglycan mRNA and expression of protein were positively correlated ( R = 0. 9991, R = 0. 9931 respectively ). Conclusion The production of collagen II and proteoglycan of the cultured fetal endplate cartilage cells were promoted by TGF-β1 in vitro, and there were significant correlation between collagen II and proteoglycan.
关键词
转化生长因子-β1/软骨终板细胞/II型胶原/蛋白多糖/胎儿Key words
Transforming growth factor-β1 /Endplate cartilage cells/ Collagen II/Proteoglycan/ Fetus引用本文复制引用
李全修,蔡月艳,刘欣,樊培新,刘勇..外源性转化生长因子-β1作用下胎儿软骨终板细胞II型胶原与蛋白多糖的相关性[J].疑难病杂志,2012,11(12):926-928,3.
