云南农业大学学报2012，Vol.27Issue(6)：809-813,5.DOI:10.3969/j.issn.1004-390X(n).2012.06.007
侵染云南烟草的番茄斑萎病毒(TSWV)的RT-PCR检测
The Detection of Tomato Spotted Wilt Virus from Tobacco by RT-PCR in Yunnan
摘要
Abstract
Tomato spotted wilt virus (TSWV) infects more kind plants and has wide host range. Recent years, it was reported that Tomato Spotted Wilt Virus infects tobacco and cause severe damage. It is very important for breeding health tobacco seeding and guiding disease control to study the detection by RT-PCR. The primers were designed according nucleocapsid N gene. The total five samples ( 102, 514, Zi10, Zi16, Zi58) suspected to be infected by TSWV were detected by RT-PCR. The PCR products were cloned into pMD vector and sequenced. The sequences of five samples showed higher than 97. 04% nucleotide sequences similarities and 97. 67% amino acid similarities. The results proved that the five samples were infected by TSWV. The detection TSWV method was constructed.关键词
番茄斑萎病毒/RT-PCR/检测Key words
Tomato spotted wilt virus/ RT-PCR/ detection分类
农业科技引用本文复制引用
佟爱仔,赵兴能,孔宝华,陈海如,蔡红,杨根华,秦西云,莫笑晗..侵染云南烟草的番茄斑萎病毒(TSWV)的RT-PCR检测[J].云南农业大学学报,2012,27(6):809-813,5.基金项目
云南省烟草公司合作项目(2009YN005,2010YN18,2010YN19). （2009YN005,2010YN18,2010YN19）
