云南农业大学学报2012，Vol.27Issue(6)：826-832,7.DOI:10.3969/j.issn.1004-390X(n).2012.06.010
日粮能量水平对乌金猪肝脏组织脂类代谢相关基因表达的影响
Effect of Dietary Energy Levels on the Expression Levels of Hepatic Lipid Metabolism Genes in Wujin Pigs
摘要
Abstract
The aim of the study was to investigate the effects of dietary energy level on expression levels of hepatic lipid metabolism genes in Wujin pigs. Fifty-four Wujin pigs with the body weight of 15 kg were randomly assigned into three groups and imposed on three different energy levels, which were low energy (DE: 11.84 MJ/kg) , middle energy ( DE: 12.97 MJ/kg) and high energy ( DE: 14. 18 MJ/kg) respectively. At the body weights of 30, 60 and 100kg, pigs were slaughtered and liver samples were taken for analyzing the expression levels of lipid metabolism genes by RT-PCR. The results showed that the expression levels of acetyl-CoA carboxylase ( ACC) , fatty acid synthase ( FAS) and sterol regulatory element binding protein 1 c ( SREBP-1c) genes in high dietary energy group were significantly higher than low and middle energy groups ( P < 0. 05 ). On the other hand, high energy could significantly reduce the expression of carnitine palmitoyltransferase I (CPT-I) , peroxisome pro-liferator-activated receptor a (PPAR α) genes ( P < 0. 05 ). This study would supply the theoretical foundation for the scientific feeding of Wujin pigs.关键词
能量水平/肝脏组织/脂类代谢/基因表达/乌金猪Key words
energy levels/ liver/ lipid metabolism/ gene expression/ Wujin pigs分类
农业科技引用本文复制引用
黄英,李永能,杨明华,潘洪彬,赵素梅,高士争..日粮能量水平对乌金猪肝脏组织脂类代谢相关基因表达的影响[J].云南农业大学学报,2012,27(6):826-832,7.基金项目
云南省自然科学基金重点项目(2005C0008Z). （2005C0008Z）
