云南农业大学学报2012，Vol.27Issue(6)：870-874,5.DOI:10.3969/j.issn.1004-390X(n).2012.06.017
大花香水月季再生体系的初步建立
Prelimary Establishment of the Regeneration System of Rosa odorata var. gigantea
摘要
Abstract
Rosa odorata var. Gigantea was used to investigate factors that effected callus induction and differentiation. The factors contained different concentration of hormone and combination, dark time and so on. The result showed that; MS medium supplied with 6-BA 1. 5 mg/L and NAA 0. 12 mg/L was suitable for inducing axillary buds. Being cultured on MS +2, 4-D 5. 0 mg/L, the induction frequency can up to 93.33%. Being cultured on MS + TDZ 3.0 mg/L + GA3 1.0 mg/L + NAA 0. 1 mg/L for 15d in darkness, the rate of adventitious bud can up to 18% . A regeneration system was established for R. Odorata var. Gigantea by indirect organogenesis, which could provide a foundation for genetic improvement for roses with biotechnology.关键词
大花香水月季/愈伤组织/分化/植株再生Key words
R. Odorata var. Gigantea/ callus/ differentiation/ plant redifferentiation分类
农业科技引用本文复制引用
孟令宁,晏慧君,张颢,蹇洪英,周宁宁,唐开学..大花香水月季再生体系的初步建立[J].云南农业大学学报,2012,27(6):870-874,5.基金项目
国家"863"计划项目(2011AA100208) （2011AA100208）
国家"948"计划项目(2011-G17) （2011-G17）
国家自然科学基金项目(31060268). （31060268）
