针刺研究2012，Vol.37Issue(5)：357-362,6.
自体血穴位注射对哮喘大鼠肺组织GATA3和T-bet蛋白及mRNA表达的影响
Effects of Acupoint Injection of Autoblood on Expression of Pulmonary Transcription Factor GATA 3 and T-bet Proteins and Genes in Asthma Rats
摘要
Abstract
Objective To observe the effect of autoblood acupoint-injection (ABAI) on expression levels of pulmonary transacting T-cell-specific transcription factor GATA 3 (involving Th 2 cytokine expression) , Th 1-specific T-box transcription factor T-box expressed in T-cells (T-bet) proteins and genes in asthmatic rats so as to explore its mechanisms underlying asthma relief. Methods Forty-eight male SD rats were randomized into normal control (n = 8), model (n = 10), saline acupoint-injection (SAI, n = 10), ABAI (n = 10), and Dexamethasone (DXM, n = 10) groups. Asthma model was established during 28 days by 10% Ovalbumin+ 10% aluminium hydroxide solution injection (i. p.) and vapourized 2% Ovalbumin inhaling for 14 days. For rats of the ABAI group, 0. 4 ml_ autoblood was injected into the bilateral "Feishu" (BL 13) or "Shenshu" (BL 23) alternately, once every other day for six times. For rats of the DXM group, 50% DXM solution (0. 5 mg/kg, i. p.) was given from the 17th day on after starting the modeling, once every other day for 11 days. Pulmonary GATA 3 and T-bet protein expression was detected by immunohistochemistry, and GATA 3 mRNA and T-bet mRNA expression detected by real time-PCR. Results In comparison with the normal group, pulmonary GATA 3 protein and mRNA expression levels in the model group were up-regulated significantly (P<0. 01), while T-bet mRNA expression in the model group was down-regulated obviously( P<0. 01). Compared to the model group, GATA 3 protein and mRNA expression levels were down-regulated significantly in both ABAI and DXM groups (P<0. 01), while T-bet protein expression in ABAI group and T-bet mRNA expression in both ABAI and DXM groups were up-regulated significantly (P<0. 01, P<0. 05). No significant differences were found between model and SAI groups, and between ABAI and DXM groups in GATA 3 protein expression levelss and between ABAI and DXM groups in GATA 3 mRNA expression levels; between normal and model groups, and between SAI and ABAI groups in T-bet protein expression levels; between model and SAI groups and between ABAI and DXM groups in T-bet mRNA expression levels (P>0. 05). The ratio of GATA 3 mRNA/T-bet mRNA expression was significantly higher in the model group than in the normal group (P<0. 01), while obviously lower in the SAI, ABAI and DXM groups than in the model group (P<0. 05, P<0. 01). Additionally, the ratios of GATA 3 mRNA/T-bet mRNA in ABAI and DXM groups were comparable (P>0. 05). Conclusion Autoblood acupoint injection is comparable to DXM intraperitoneal injection in down-regulating asthma-induced increase of pulmonary GATA 3 protein and mRNA expression as well as ratio of GATA 3 mRNA/T-bet mRNA, and in up-regulating asthma-induced decrease of T-bet mRNA expression in asthma rats, which may contribute to their effects in relieving asthma.关键词
哮喘/自体血穴位注射/GATA 3/T-bet/GATA 3/mRNA/T-bet mRNAKey words
Asthma/Autoblood acupoint-injection/Th 2-specific transcription factor GATA 3/Th 1-specific transcription factor T-bet/GATA 3 mRNA/T-bet mRNA分类
医药卫生引用本文复制引用
莫律,李俊雄,Julia Kravitz,唐纯志,郭泽楷,李培勇,郭凤美..自体血穴位注射对哮喘大鼠肺组织GATA3和T-bet蛋白及mRNA表达的影响[J].针刺研究,2012,37(5):357-362,6.基金项目
佛山市医学类科技攻关项目(201008125) （201008125）
