针刺研究2012，Vol.37Issue(5)：380-384,5.
电针对脑缺血再灌注大鼠脑红蛋白的影响
Involvement of Cerebral Neuroglobin in Electroacupuncture Preconditioning-induced Protection Effect in Cerebral Ischemia-reperfusion Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) preconditioning on cerebral ischemia and the role of cerebral neuroglobin (NgB) in EA-induced brain protection in focal cerebral ischemia and reperfusion injury (CI/RI) rats. Methods Male SD rats were randomly assigned to sham control, CI/RI 6 h, CI/RI 24 h and CI/RI 72 h groups (n = 6) for observing changes of NgB at different time-points. Additional SD rats were randomly assigned to sham, model, and EA preconditioning (EA-PC) groups (n = 16) for observing changes of cerebral NgB positive cell counts in the iscnemic penumbra region 24 h after reperfusion. EA pre-conditioning was applied to "Baihui" (GV20) for 30 min, once daily for 5 days before CI/RI. CI/RI model was established by occlusion of the right middle cerebral artery and reperfusion for 6 h, 24 h and 72 h respectively. The neurological behavior scores (NBS) of all the rats were evaluated according to Garcia's methods. The cerebral infarct volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The number of cerebral NgB positive cells was detected by immuno-fluorescent staining. Results No infarct loci were found in the sham group. The cerebral infarction volume percentage was significantly higher in the model group than in the EA-PC group(P<0. 01), while the NBS was significantly lower in the model group than in the EA-PC group (P<0. 01). The number of cerebral NgB positive cells in the ischemic penumbra was up-regulated 6 h after CI/RI injury, peaked at 24 h and continued at 72 h. Compared with the sham group, the number of cerebral NgB positive cells of the model group was increased significantly, whereas that of the EA-PC group up-regulated further obviously in comparison with the model group (P<0. 01) . Conclusion EA pretreatment has a significant neuroprotective effect on cerebral ischemia-reperfusion, which is closely related to its effect in up-regulating NgB protein expression.关键词
脑缺血再灌注/电针预处理/脑红蛋白/神经保护Key words
Cerebral ischemia-reperfusion/Electroacupuncture preconditioning/Cerebral neuroglobin/Neuropro-tection分类
医药卫生引用本文复制引用
谢亚宁,王枫,王强,李旭颖,张巧梅,李新,熊利泽..电针对脑缺血再灌注大鼠脑红蛋白的影响[J].针刺研究,2012,37(5):380-384,5.基金项目
国家自然科学基金资助项目(No.81072888、No.30900462) （No.81072888、No.30900462）
