针刺研究2012，Vol.37Issue(6)：458-463,6.
电针治疗肠黏膜损伤大鼠基本腧穴配伍“肠病方”的筛选
Selection of Basic Acupoints Combined with "Enteral-Disorder-Formuia" for Electroacupuncture Treatment of Intestinal Mucosal Lesion in Rats
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) stimulation of differently combined acupoints of "Zhongwan"(CV 12), "Tianshu"(ST 25) and "Shangjuxu" (ST 37, for treatment of enteral disorders) on acetic acid-induced intestinal mucosai lesion in rats so as to select the best combinations oi acupoints. Methods A total of 126 SD rats were divided into nine groups: normal control (normal), model, CV 12, ST 25, ST 37, CV 12 + ST 37, ST 25+ ST 37, CV 12 + ST 25 and CV 12 +ST 25 +ST 37. Intestinal mucosal lesion model was established by using enema (8% acetic acid 1 mL). EA was applied to the above-mentioned acupoints for 15 min, once daily for 3 days. General conditions, colonic mucosal damage index (CMDI) and pathological damage integral (PDI) were detected respectively. The colonic mucosal membrane tissue was stained with H.E. stain for observing its morphological changes under light microscope, and the ultrastructural changes were observed using electron transmission microscope. Results Compared with the control group, CMDI was decreased significantly (P<0. 05) and PDI of the colonic mucosal membrane increased significantly in the model group (P<0. 05). Compared with the model group, CMDI was decreased markedly and PDI increased significantly (P<0. 05) in the CV 12, ST 25, ST 37, CV 12 + ST 37, ST 25 + ST 37, CV 12 + ST 25 and CV 12 + ST 25 + ST 37 groups (P<0. 05). Comparison among the 7 EA treatment groups showed that both CMDI and PDI of the CV 12 + ST 25 + ST 37 group were significantly superior to those of CV 12, ST 25, ST 37, CV 12 + ST 37, ST 25 + ST 37 and CV 12 + ST 25 groups (P<0. 05). Under light microscope, the colonic mucosal injury was remarkable, while those of the 7 EA treatment groups were relatively milder. Ultramicrostructural findings showed that the colonic mucosal lesion including shortening or sparseness of the intestinal epithelial villi, swelling of mitochondria with arrangement disorder, breaking or fusion of the cristae, and enlargement of the rough endocytoplasmic reticulum was found in the model group, while the situations were lighter in the 7 EA groups particularly in the CV 12 + ST 25 + ST 37 group. Conclusion EA of CV 12, ST 25 and ST 37 acupoint alone or combination can relieve acetic acid-induced intestinal mucosal lesion in rats, and the effect of joint application of these 3 acupoints is obviously better, suggesting their clinical applicability in clinical treatment of intestinal disorders as a basic formula.关键词
肠黏膜损伤/电针/腧穴配伍Key words
Intestinal mucosal lesion/Electroacupuncture/Acupoints combination分类
医药卫生引用本文复制引用
闫丽萍,冀来喜,王海军,程艳婷,燕平,李明磊,赵霞..电针治疗肠黏膜损伤大鼠基本腧穴配伍“肠病方”的筛选[J].针刺研究,2012,37(6):458-463,6.基金项目
国家自然科学基金资助(No.30973799) （No.30973799）
