中国病理生理杂志2012，Vol.28Issue(12)：2130-2134,5.DOI:10.3969/j.issn.1000-4718.2012.12.004
低硒大鼠心肌线粒体硫氧还蛋白2的动态表达和心肌的损伤情况
Dynamic expression of thioredoxin 2 in myocardial mitochondria in selenium-deficiency rats during myocardial injury
摘要
Abstract
To investigate the dynamic expression of thioredoxin 2 (Trx2) protein in the myocardial mitochondria under the condition of selenium deficiency . METHODS: A model of selenium deficiency was made using two -week - old SD rats. When the rats were fed for 20 weeks, 30 weeks and 40 weeks, the cardiac functions were detected by carotid artery intubation. The myocardial mitochondria were also extracted , and the protein expression of mitochondrial Trx 2 was measured by Western blotting. The method of immunohistochemistry was used to detect Fas -associated death domain protein ( FADD, a death marker protein ) for determining the apoptosis of myocardial cells . The correlation between Trx2 and left ventricular systolic pressure ( LVSP) , maximum rate of left ventricular pressure rise ( + dp/dimax) and apoptotic index of the myocardial cells was analyzed . RESULTS: Compared with the corresponding period of normal control group , the expression of Trx2 protein in the selenium -deficiency rats was reduced. The expression of Trx2 protein was continuously reduced as the time of selenium deficiency prolonged . As the time of low -selenium feeding was going on , the systolic and diastolic functions of the heart were impaired , and the number of apoptotic myocardial cells was increased . The correlation analysis indicated that Trx2 had positive correlations with LVSP and + dp/dtnax, and had a negative correlation with the ap -optotic index of myocardial cells . CONCLUSION: Selenium deficiency affects the expression of Trx 2 protein, and causes impaired cardiac functions and the apoptosis of myocardial cells . Trx2 has a protective effect on myocardial cells.关键词
心肌线粒体/硫氧还蛋白2/心功能/细胞凋亡Key words
Myocardial mitochondria/Thioredoxin 2/Cardiac function/Apoptosis分类
医药卫生引用本文复制引用
潘晓青,魏瑾,张明,林琳,单虎,闫蕊,贺乐..低硒大鼠心肌线粒体硫氧还蛋白2的动态表达和心肌的损伤情况[J].中国病理生理杂志,2012,28(12):2130-2134,5.基金项目
国家自然科学基金资助项目(No.30771862) （No.30771862）
陕西省科技攻关项目[No.2007K13-03(14)] （14）
