中国病理生理杂志2012，Vol.28Issue(12)：2205-2210,6.DOI:10.3969/j.issn.1000-4718.2012.12.017
不同剂量骨髓间充质干细胞移植对大鼠矽肺纤维化形成的影响
doi:10.3969/j.issn.1000-4718.2012.12.017Effects of bone mesenchymal stem cell transplantation on silicosis fibrosis in rats
摘要
Abstract
ATM; To study the effects of exogenous bone mesenchymal stem cell (BMSC) transplantation on silicosis fibrosis in rats, and to explore the dose - effect relationship. METHODS: BMSCs were isolated and cultured from male 5 - week - old SD rats in vitro. Fifty healthy female SD rats were randomly divided into 5 groups : control group , silicosis model group , BMSCs treatment A group (1x10 cells/L) , BMSCs treatment B group (3x10 cells/L) and BMSCs treatment C group (5 X 109 cells/L). The silicosis model was made by one -time infusion of silica dust suspension using the non - exposed tracheal intubation , and different doses of BMSCs were given for intervention therapy . All the rats were sacrificed on the 21st day after the model was established. The morphological changes of the lung tissues were observed by HE staining and Masson staining. The localization and distribution of tumor necrosis factor a (TNF - α) and transforming growth factor β (TGF - β) were determined by the method of immunohistochemistry . The protein levels of TNF - α, TGF - β, collagen type I and collagen type III were detected by Western blotting . The sex - determining region ( SRY) protein was searched by an immunofluorescence method to confirm the homing of BMSCs . RESULTS; Compared with control group, the silicosis model group had significant alveolitis changes , silicon nodule formation, collagen deposition and other pathological characteristics. Compared with silicosis model group, the pathological changes in BMSCs treatment A group were improved. The conditions of BMSCs treatment B group were also improved significantly . However,the pathological changes in BMSCs treatment C group were increased obviously . The protein levels of TNF -α, TGF - β, collagen type I and collagen type III in the lung tissues ranked as follows : BMSCs treatment C group > silicosis model group > BMSCs treatment A group > BMSCs treatment B group > control group. The difference between BMSCs treatment C group and sil -icosis model group was not statistically significant , and the differences between the other groups were statistically signifi -cant. The SRY -positive cells were observed in BMSCs treatment B group , but no significant expression in the heart , liver, spleen and kidney tissues was observed . CONCLUSION; The exogenous BMSC transplantation antagonizes the devel -opment of silicosis fibrosis in rats , which has dose -effect relationship.关键词
矽肺/骨髓间充质干细胞/Ⅰ型胶原/Ⅲ型胶原/肿瘤坏死因子α/转化生长因子βKey words
Silicosis/Bone mesenchymal stem cells/Collagen type I/Collagen type III/Tumor necrosis factor alpha/Transforming growth factor beta分类
医药卫生引用本文复制引用
赵曼曼,崔建忠,李冉,都凌杰,田艳霞,阚泉,张娟,高俊玲..不同剂量骨髓间充质干细胞移植对大鼠矽肺纤维化形成的影响[J].中国病理生理杂志,2012,28(12):2205-2210,6.基金项目
河北省科技支撑重点专项基金资助项目(No.09276191D) （No.09276191D）
国家安全生产监督管理局科技发展计划资助项目(No.06-549) （No.06-549）
