中国病理生理杂志2012，Vol.28Issue(12)：2238-2243,6.DOI:10.3969/j.issn.1000-4718.2012.12.023
氧化应激在糖尿病大鼠胃动力变化中的意义
Role of oxidative stress in gastric motility of diabetic rats
摘要
Abstract
ATM: To investigate the changes of oxidative stress in the stomach tissues and their roles in gastric motility and interstitial cells of Cajal (ICC) in diabetic rats. METHODS; Thirty - eight SD rats (8-week-old, male) were intraperitoneally injected with streptozotocin ( STZ). Diabetes was successfully induced in 36 of them. The diabetes rats were randomly divided into untreated diabetes group and treated diabetes group . Eighteen healthy SD rats (8 -week -old, male) served as controls. The body weight and the levels of blood glucose and glycosylated hemoglobin were meas -ured. At the end of week 1 and week 10, 9 rats were sacrificed in each group. The gastric emptying rate and the levels of malondialdehyde (MDA) ,superoxide dismutase (SOD), tumor necrosis factor a (TNF-α), tyrosine kinase receptor c - Kit and stem cell factor ( SCF ) in gastric smooth muscle were analyzed. The apoptosis of ICC in gastric tissues was de -tected by the methods of immunocytochemistry and TUNEL . RESULTS: Compared with control group , gastric motility and SOD activity in untreated diabetes group were significantly weakened , the levels of MDA and TNF - a increased, the levels of c - Kit and SCF decreased , and apoptosis of ICC enhanced . In treated diabetes group , the oxidative stress level was at -tenuated, antioxidant capacity was enhanced , the levels of c - Kit and SCF were significantly increased , and the ICC apoptosis was reduced. Gastric motility was significantly improved after antioxidant therapy . CONCLUSION; Hyperglycemia affects the expression of antioxidant enzymes in the stomachs of diabetic rats . Oxidative stress is caused by hyperglycemia and is an important factor in the etiology of gastric motility dysfunction in diabetic rats , which may be correlated with the augmentation of ICC apoptosis resulting from oxidative stress - induced c - Kit/SCF damage.关键词
糖尿病/氧化性应激/胃动力Key words
Diabetes mellitus/Oxidative stress/Gastric motility分类
医药卫生引用本文复制引用
金启辉,管文花,王辉,朱延涛,陈怀红,娄域峰,陈瑛..氧化应激在糖尿病大鼠胃动力变化中的意义[J].中国病理生理杂志,2012,28(12):2238-2243,6.基金项目
浙江省卫生厅项目(No.N20100767) （No.N20100767）
浙江省中医药科技计划项目(No.2010ZB076) （No.2010ZB076）
