中国病理生理杂志2012，Vol.28Issue(12)：2301-2304,4.DOI:10.3969/j.issn.1000-4718.2012.12.036
应用亚硫酸钠快速制备低氧性肺血管收缩离体模型所需低氧性Krebs-Henseleit液的方法及评价
Rapid preparation of hypoxic Krebs-Henseleit solution with sodium sulfite for in vitro model of hypoxic pulmonary vasoconstriction
摘要
Abstract
ATM: To investigate the feasibility of using sodium sulfite ( Na2SO, ) to prepare hypoxic Krebs -Henseleit (KH) solution for hypoxic pulmonary vasoconstriction (HPV) model in vitro. METHODS; Different doses of Na2SO, were added into 0. 5 L KH solution at 37 ℃ . An i - STAT portable clinical analyzer was used to measure the oxy -gen partial pressure ( PO2) , carbon dioxide partial pressure ( PCO2) , pH value and the concentration of sodium ( Na + ) in these KH - Na2SO, solutions 1 min after administration. Then the dose of Na2SO, suitable for HPV model was dissolved in 0. 5 L KH solution and the above indexes in the solution were monitored at various time points at 37 X. under atmospheric pressure. RESULTS: More than 0. 2 g (including 0. 2 g) Na2SO, reduced the PO2 of 0. 5 L KH solution in a dose - dependent manner ( P < 0. 01 ) . In addition, 1.5 g Na2 SO, reduced the PO 2 of 0. 5 L KH solution to20~40mmHgand maintained the hypoxic state for at least 90 min ( suitable for HPV model in vitro) , but had nearly no effect on the PCO2, pH and Na levels. CONCLUSION; The hypoxia solution for HPV model could be reached by Na 2SO, in open air and the method is simple , easily feasible and stable.关键词
Krebs-Henseleit液/亚硫酸钠/低氧性肺血管收缩Key words
Krebs - Henseleit solution/Sodium sulfite/Hypoxic pulmonary vasoconstiction分类
医药卫生引用本文复制引用
姚海霞,祝卿,吉伟,王淑君,王良荣,林丽娜,王万铁..应用亚硫酸钠快速制备低氧性肺血管收缩离体模型所需低氧性Krebs-Henseleit液的方法及评价[J].中国病理生理杂志,2012,28(12):2301-2304,4.基金项目
浙江省自然科学基金(No.YZ080760) （No.YZ080760）
卫生部科学研究基金 (No.WKJ2009-2030) （No.WKJ2009-2030）
浙江省温州市科技合作项目(No.HZ0090066) （No.HZ0090066）
浙江省公益性技术应用研究计划项目(No.2012C37092) （No.2012C37092）
