中国当代儿科杂志2012，Vol.14Issue(11)：811-814,4.
198例哮喘儿童遗传易感性研究
Study of genetic susceptibility in 198 children with asthma
郑伯强 1王桂兰 1杨赛 1鲁衍强 2刘睿俊 2李瑛2
作者信息
- 1. 中山市博爱医院儿科,广东 中山 528403
- 2. 上海靶向分子医学研究所,上海 200433
- 折叠
摘要
Abstract
Objective To analyze the frequency distribution of single nucleotide polymorphisms ( SNPs) of four asthma-related gene loci (ACE I/D; ADRB2 Argl6Gly; TNF-α G-308A; MS4A2 Glu237Gly) in 198 asthmatic children, and to investigate its association with genetic susceptibility to childhood asthma and some clinical phenotypes of asthma. Methods Polymerase chain reaction product electrophoresis identification and real-time quantitative PCR detecting system were used to determine the frequency distributions of the SNPs of the four asthma-related gene loci in 198 asthmatic children and 110 healthy controls. The serum total IgE (TIgE) levels and blood eosinophil proportion (%EOS) of the asthmatic children were measured. Different genotypes at the four asthma-related gene loci were compared in terms of TIgE and %EOS. Results The genotype DD of angiotensin-converting enzyme (ACE) had a significantly higher frequency in the asthmatic children than in the healthy controls (x2 = 30. 667, P < 0. 01 ) , and the frequency of D allele was also significantly higher in the asthmatic children than in the healthy controls (x2 =7. 151, P < 0. 01 ). No correlation was found between the polymorphism of each gene locus and serum TIgE level and %EOS (P > 0. 05). Conclusions Genotype DD of ACE is related to genetic susceptibility to childhood asthma and may be the risk factor for childhood asthma.关键词
哮喘/单核苷酸多态性/遗传易感性/儿童Key words
Asthma/Single nucleotide polymorphism/Genetic susceptibility/Child分类
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郑伯强,王桂兰,杨赛,鲁衍强,刘睿俊,李瑛..198例哮喘儿童遗传易感性研究[J].中国当代儿科杂志,2012,14(11):811-814,4.
