中国当代儿科杂志2012，Vol.14Issue(12)：971-975,5.
骨髓间充质干细胞移植对视网膜病变新生大鼠视网膜细胞凋亡的影响
Effects of bone marrow mesenchymal stem cell transplantation on retinal cell apoptosis in premature rats with retinopathy
摘要
Abstract
Objective To explore the effects of marrow mesenchymal stem cell (BMSC) transplantation on retinal cells apoptosis and changes to neurotrophin-3 (NT-3 and ciliary neurotrophic factor (CNTF) in rats with retinopathy of prematurity (ROP). Methods Seven-day-old Sprague-Dawley rats were randomly divided into normal control (CON), ROP, BMSC transplantation (BMSCs were transplanted 5 days after oxygen conditioning) and phosphate buffered saline (PBS) groups. The ROP model was prepared according to the classic hyperoxygen method. Seven days after transplantation, TUNEI/DAPI, NT-3/ API and CNTF/DAPI double-labeled immunofluorescence were used to examine the effects of BMSC transplantation on both the apoptosis of retinal cells and the expression of NT-3 and CNTF protein in the retinal cells of the ROP rats. Results Seven days after BMSC transplantation, there were few TUNEL+ DAPI+ cells observed in the CON group. There were fewer TUNEL+ DAPI + cells observed in the BMSC group than in the ROP group ( P < 0.01) , but there was no significant difference between the ROP and PBS groups ( P > 0.05 ). There were few NT-3 + DAPI + cells and CNTF + DAPI + cells in the CON group. There were more NT-3+ DAPI+ and CNTF+ DAPI+ cells in the ROP group than in the CON group, but there was no significant difference between the ROP and CON groups (P > 0.05 ). More NT-3 + DAPI + and CNTF + DAPI + cells were observed in the BMSC group compared with the ROP group ( P < 0. 01) , and there was no significant difference in either NT-3 +DAPI+ or CNTF + DAPI + cells between the ROP and PBS groups ( P > 0. 05 ). Conclusions BMSC transplantation therapy could alleviate the apoptosis of retinal cells in ROP rats, and its mechanisms might be associated with promoting the expression of NT-3 and CNTF protein in retinal cells.关键词
视网膜病变/骨髓间充质干细胞移植/凋亡/神经营养素-3/睫状神经营养因子/大鼠Key words
Retinopathy/Bone marrow mesenchymal stem cell transplantation/Apoptosis/Neurotrophin-3/Ciliary neurotrophic factor/Rats分类
医药卫生引用本文复制引用
赵岩松,赵堪兴,王晓莉,陈玉玺,王丽,牟青杰..骨髓间充质干细胞移植对视网膜病变新生大鼠视网膜细胞凋亡的影响[J].中国当代儿科杂志,2012,14(12):971-975,5.基金项目
国家自然科学基金项目(81000268) （81000268）
山东省自然科学基金项目(ZR2010HQ037) （ZR2010HQ037）
山东省教育厅课题(J11LF72). （J11LF72）
