中国动脉硬化杂志2012，Vol.20Issue(10)：881-884,4.
Valsartan延缓血管内皮细胞衰老及p16INK4a表达变化的研究
Study on Valsartan Delaying Endothelial Cell Senescence and Gene Expression of p16INK4a
摘要
Abstract
Aim To investigate the role of Valsartan on Angiotensin Ⅱ ( Ang Ⅱ )-induced senescence of human umbilical endothelial cell senescence and gene expression of pl6INK4a. Methods HUVEC were cultured in vitro and intervened by Ang Ⅱ (l0‐6mol/L) and Valsartan (Ang Ⅱ type 1 receptor blocker). HUVEC were divided into 3 groups, the control group, Ang Ⅱ group, Valsartan group. β-gal staining was used to identify cell aging status. Flow cytometry was used for analyzing the cell cycle changes; The positive cell rate of pl6 INK4a was detected by immunocytochemical staining, and the expressions of pl6 INK4a protein were determined by Western blot. Results Compared with the control cells, the positive cell number of β-gal staining was significantly higher in AngⅡ -induced cells 81. 24% ±6. 46% ; the cell cycle was at Go-G, 88.36% ±6.45%. In Valsartan group, pl61NK4β protein expression decreased evidently (P < 0. 05) compared to that in the Ang Ⅱ group, which suggests thaf pl61NK4β activity plays an important role in regulating vascular endothelial cell senescence lifespan in vitro. Conclusion Cell Endothelial cell senescence is induced by Ang Ⅱ . One of its molecular mechanisms might be associated with increasing the expression level of pl6INK4a in aging cell, and then up-regulating the amount of cells blocking in G, phase of cell cycle. Valsartan could antagonize the process effectively and delay endolhelial cell aging significantly.关键词
血管紧张素Ⅱ/内皮细胞/血管衰老/细胞周期/p16INK4aKey words
Valsartan/ Angiotensin II / Endothelial Cell/ Vascular Aging/ Cell Cycle/ P16INK4a分类
医药卫生引用本文复制引用
单海燕,白小涓,王鹤智,陈香美..Valsartan延缓血管内皮细胞衰老及p16INK4a表达变化的研究[J].中国动脉硬化杂志,2012,20(10):881-884,4.基金项目
国家自然科学基金资助项目(81200245) （81200245）
中华医学会临床医学科研专项资金-动脉粥样硬化研究资金资助(09010530208) （09010530208）
辽宁省科学技术研究项目(201202263) （201202263）
沈阳市科学技术计划项目(F10-205-1-44) （F10-205-1-44）
