中国动脉硬化杂志2012，Vol.20Issue(11)：995-998,4.
三种剂量维生素D3结合高脂饲料建立大鼠动脉粥样硬化模型的比较
Comparison of Methods for Establishing A Rat Model of Atherosderosis Using Three-doses of Vitamin D3 and Atherogenic Diet
摘要
Abstract
Aim To explore an efficient method to establish a rat model of atherosclerosis using vitamin D3 ( VitD3) and atherogenic diet. Methods Forty healthy Wistar rats were randomly divided into 4 groups, ie, a control group receiving standard diet, three model groups receiving atherogenic diet for 12 weeks. At the same time, model group 1 were injected VitD3 7. 0 × 105 IU/kg every two days before feeding for four times; model group 2 were injected VitD3 6. 0 × 105 IU/kg before feeding and 1. 0 × 105 IU/kg in the third, sixth and ninth week respectively after feeding; model group 3 were injected VitD3 6. 0 × 105 IU/kg before feeding and in the third,sixth and ninth week respectively after feeding. The body weights of all rats were recorded between 12 weeks. After 12 weeks, total cholesterol(TC) , high-density lipoprotein cholesterol (HDLC) , low-density lipoprotein cholesterol ( LDLC ), and triacylglycerol (TG) were measured. HE staining was used to verify the atherosclerotic lesion in aorta. Results Compared with the control group, the body weights of model groups were seriously decreased (P <0. 01) , especially model group 3 and its high death rate. The serum levels of TC and LDLC in model groups were obviously increased than the control group. The numbers in three model groups producing typical atherosclerotic lesion in aorta were 3 , 8 and 5 respectively. Conclusions It was an effective method for establishing a rat model of atherosclerosis by feeding atherogenic diet and injecting VitD3, and the suitable dose of VitD3 was 6 x 10s 1U /kg before feeding and 1. 0 × 105 IU /kg in the third, sixth and ninth week respectively after feeding.关键词
动脉粥样硬化/动物模型/维生素D3Key words
Atherosclerosis/ Animal Model/ Vitamin D3分类
医药卫生引用本文复制引用
周红,吴晓燕,袁艺标,戚晓红..三种剂量维生素D3结合高脂饲料建立大鼠动脉粥样硬化模型的比较[J].中国动脉硬化杂志,2012,20(11):995-998,4.基金项目
南京医科大学基础医学院优势学科科研创新配套经费(JX10131801095) （JX10131801095）
