中国动脉硬化杂志2012，Vol.20Issue(12)：1057-1063,7.
Calphostin C抑制脂肪分化相关蛋白诱导的ACAT1表达
Calphostin C Inhibit Adipophilin-induced Expression of ACAT 1
摘要
Abstract
Aim To investigate the signaling pathways involved in the process which adipophilin induced the in-tracellular lipid accumulation. Methods We obtained the RAW264. 7 cells of high or low expression of adipophilin by constructing retroviral vector. Then the cells were incubated with 300 nmol/L of Calphostin C (PKC inhibitor) for 16 h or incubated with 50 mg/L ox-LDL simultaneously.The expression of ACAT1 was detected by RT-PCR and Western blot.Results The results showed that RAW264. 7 cells with high expression of adipophilin increased the expression of ACAT1, and decreased in cells of low expression of adipophilin. Calphostin C inhibited the expression of ACAT1 in the cells with high or low expression of adipophilin. Compared with the cells incubated without Calphostin C, the difference was significant. Calphostin C also inhibited the expression of AC ATI when incubated the cells of high expression of adipophilin with ox-LDL for lipid-loading. Both mRNA and protein were significantly decreased after incubated for 16 h and the difference was significant compared with the control. In the condition of lipid-loaded, Calphostin C could inhibit ACAT1 expression time-dependently in cells of low expression of adipophilin. Conclusion The results indicated that PKC signaling molecules affected adipophilin expression and further affected the expression of ACAT1. It suggests that PKC signal could be related to adipophilin causing intracellular lipid accumulation.关键词
脂肪分化相关蛋白/乙酰辅酶A:胆固醇酰基转移酶1/Calphostin CKey words
Adipophilin/ Acyl-coenzyme A: Cholesterol Acyltransferse 1/ Calphostin C分类
医药卫生引用本文复制引用
袁中华,陶媛,刘清南,黄谙非,刘晓辉,田国平,唐朝克,易光辉,王佐..Calphostin C抑制脂肪分化相关蛋白诱导的ACAT1表达[J].中国动脉硬化杂志,2012,20(12):1057-1063,7.基金项目
国家自然科学基金(30971268) （30971268）
教育部留学回国人员科研启动基金(教外司留[2008]890号) （教外司留[2008]890号）
