中国动脉硬化杂志2012，Vol.20Issue(12)：1074-1078,5.
小檗碱调节肝X受体α去乙酰化促进THP-1巨噬细胞ATP结合盒转运体A1表达及其胆固醇流出
Berberine Increases the Expression of ATP-Binding Cassette Transporter A1 and Cholesterol Efflux in THP-1 Macrophages via Deacetylation of Liver X Receptor-α
摘要
Abstract
Aim To investigate the effect and mechanism of berberine on ATP-binding cassette transporter Al (ABCA1) expression and cholesterol efflux in THP-1 macrophage. Methods THP-1 macrophage foam cell were exposed to berberine with different levels(0, 5, 10, 20 μmol/L) for 24 h or treated with berberine (20 μmol/L) for increasing periods of time( 0, 6, 12, 24 h). Western Blot was used to determine protein levels for ABCA1, liver X receptor-α ( LXR-α) and acetylated LXR-α. Cellular lipid accumulation was determined by high performance liquid chromatography analysis. Cholesterol efflux was determined by liquid scintillator. Results Compared with control group, berberine increased the expression of ABCA1 in dose (0-20 μmol/L) and time(0 ～ 24 h) dependent manner while decreased the acetylation of LXR-α. In addition, berberine increased the levels of cholesterol efflux and decreased the accumulation of cholesterol in THP-1 macrophage foam cells when the content of the berberine was 20 μmol/L and treated time was 24 h, the blends had the maximum impact. Conclusion Berberine may upregulate ABCA1 expression and cholesterol efflux via deacetylation of LXR-α.关键词
小檗碱/肝X受体/ATP结合盒转运体A1/胆固醇流出Key words
Berberine/ Liver X Receptor-a/ ATP-Binding Cassette Transporter Al/ Cholesterol Efflux分类
医药卫生引用本文复制引用
邓翔,尹凯,涂剑,李金凤,陈五军,肖新华,姜志胜,唐朝克..小檗碱调节肝X受体α去乙酰化促进THP-1巨噬细胞ATP结合盒转运体A1表达及其胆固醇流出[J].中国动脉硬化杂志,2012,20(12):1074-1078,5.基金项目
国家自然科学基金(81070220、81170278和81100213) （81070220、81170278和81100213）
湖南省自然科学衡阳市联合基金(10JJ9019) （10JJ9019）
湖南省研究生科技创新基金(CX2010B379) （CX2010B379）
