中国动脉硬化杂志2012，Vol.20Issue(12)：1093-1096,4.
氨氯地平下调内皮细胞源性PDGF-B表达抑制平滑肌细胞增殖和迁移
Amolodipine Inhibites the Cell Proliferation and Migration of HUASMC by Down-regulating Platelet-derived Growth Factor-B Expression Derived from HUVEC-12 Cells
摘要
Abstract
Aim To investigate the effect of platelet-derived growth factor-B ( PDGF-B) expression derived from human umbilical vein endothelial cell-12 ( HUVEC-12) on the cell migration of human umbilical arterial smooth muscle cells (HUASMC). Methods HUVEC-12 cells were pretreated with different concentrations of amlodipine (0, 0. 1, 1.0, 10. 0 μmol/L) for 30 min and then incubated with 50 mg/L oxidized low density lipoprotein (ox-LDL) for 24 h. PDGF-B protein expression was detected by enzyme-linked immunosorbent assay (ELISA) in the culture medium. HUASMC were pretreated with 10.0 μmnol/L amlodipine or 20 μmol/L PDGF-B antibody for 30 min respectively and then incubated with PDGF-B suspematant fluid for 24 h, the cell proliferation was determined by thiazolyl blue (MTT) and the migration was determined by Transwell migration experiment. Results Amlodipine could down-regulate the PDGF-B protein expression in HUVEC-12 cells induced by ox-LDL. The cell proliferation and migration were both significantly decreased in the amlodipine pretreated group, the same as PDGF-B antibody pretreated group. Conclusion Amlodipine inhibits HUASMC proliferation and migration by down-regulating endothelial cell-derived PDGF-B expression.关键词
氨氯地平/人脐静脉内皮细胞/血小板源性生长因子B/人脐动脉平滑肌细胞/细胞增殖和迁移Key words
Amolodipine/ Human Umbilical Vein Endothelial Cell-12/ Platelet-derived Growth Factor-B/ Human Umbilical Arterial Smooth Muscle Cells/ Cell Proliferation and Migration分类
医药卫生引用本文复制引用
何谨,孟军,白洁,涂剑,许选选,王佐,涂玉林..氨氯地平下调内皮细胞源性PDGF-B表达抑制平滑肌细胞增殖和迁移[J].中国动脉硬化杂志,2012,20(12):1093-1096,4.基金项目
湖南省卫生厅科技项目(B2007101)和博士启动基金(B2011XQD44)资助 （B2007101）
