摘要
Abstract
Background and objective Raf kinase inhibitory protein (RKIP) belongs to the phosphatidylethanol-amine binding protein family. RKIP is an endogenous inhibitor of the ERK/MAPK, NF-kB, and G protein-coupled receptor signaling pathways. This study aims to investigate the expression of RKIP in non-small cell lung cancer (NSCLC) and to determine the association of RKIP expression with the clinicopathologic features of NSCLC. Methods Reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry were used to detect RKIP expression in 83 specimens with NSCLC and normal lung tissues and to analyze the association of RKIP expression with the clinicopathologic features of NSCLC. All cases were confirmed by pathological diagnosis, and primary tumors were not found at other sites. No medical records of preoperative radiotherapy, chemotherapy, and immunotherapy were found in the groups. Results RKIP expression was down-regulated significantly in NSCLC compared with adjacent cancer tissues (P<0.05). It was associated with differentiation, pathological tumor-node-metastasis stage, survival time, and lympho invasion (P<0.05) but not with gender, smoking status, age, tumor size, and histologic type (P>0.05). Conclusion The deficiency of RKIP expression is positively correlated with carcinogenesis and invasion metastasis of NSCLC. RKIP is a potential marker and target for clinic therapy.关键词
肺肿瘤/Raf激酶抑制蛋白/Western blot/RT-PCR/免疫组织化学Key words
Lung neoplasms/Raf kinase inhibitory protein/Reverse transcription-polymerase chain reaction/Western blot/Immunohistochemistry分类
医药卫生
