中国科学院研究生院学报2012，Vol.29Issue(6)：847-852,6.
GST-β-catenin-His双标签融合蛋白的原核表达、纯化及鉴定
Prokaryotic expression, purification, and identification of GST-β-catenin-His double labeled fusion protein
摘要
Abstract
The cDNA sequence of p-catenin was amplified through RT-PCR with 6xHis tag added in the gene downstream, cloned into the vector pGEX-4T-l. The plasmid transformed into BL21pLysS was induced by IPTG. The induced fusion protein was purified stepwise using Glutathione Sepharose 4B and Ni columns. The final protein ran on SDS-PAGE with a specific band at the expected size of 114 kDa. Western Blotting showed that the purified protein can be recognized by the anti-β-catenin, anti-GST, and anti-His antibodies, respectively. All these results provide a basis for further study of p-catenin function.关键词
β-连环蛋白/纯化/GST-His双标签/Western BlottingKey words
β-catenin/ purification/ GST and His tags/ Western Blotting分类
医药卫生引用本文复制引用
尹会龙,袁莉..GST-β-catenin-His双标签融合蛋白的原核表达、纯化及鉴定[J].中国科学院研究生院学报,2012,29(6):847-852,6.基金项目
国家自然科学基金(Y11101L1A1)和中国科学院研究生院院长基金(095101GN00)资助 （Y11101L1A1）
