中国临床药理学与治疗学2012，Vol.17Issue(11)：1216-1222,7.
蛹虫草提取物对高糖诱导内皮细胞衰老的影响
Effect of the extract of cordyceps militaris on senescence in cultured human umbilical vein endothelial cells treated with high glucose
摘要
Abstract
AIM: To determine the effect of the ethyl acetate extract of cordyceps militaris (CME) on senescence in cultured human umbilical vein endothelial cells ( HUVECs) treated with high glucose. METHODS: The HUVECs senescence model was established by incubation with high glucose (40 mmol/L) , the extract of Cordyceps Sinensis(CSE) (50 μg/mL) was treated as a positive control, and then CME (12. 5 -100 μg/mL) was treated to observe its effect on cell senescence. Cell proliferation was determined by the methylthiazolyl tetrazolium (MTT) reduction assay , SA-β-galactosidase (SA-β-gal) positive cells were detected by X-gal, and phase distribution of cell cycles and cellular reactive oxygen species (ROS) were detected by flow cytometry. RESULTS: Compared with un-intervented cells, cell OD value significantly decreased in cells treated with high glucose for 24, 48, and 72 h (P<0. 01). The percentage of cells positive for SA-β-gal, the percentage in G0/G1 phase and ROS levels notably increased after cells incubated by high glucose for 96 or 48 h (P <0. 01). Compared with high glucose intervention cells, cell OD value significantly increased in cells intervented by CME (25 - 50 μg/mL) for 48 h and 72 h ( P<0. 05); CME(12. 5-100 μg/ mL) stimulating for 96 h significantly lowered the percentage of cells positive for SA-μ-gal (P <0. 01); CME (12. 5-100 μg/mL) incubation for 48 h significantly lowered the percentage of G0/G1 phase of HUVECs (P<0. 01) and ROS production (P<0. 05) , All these effects of CME 25, 50 μg/mL groups were better than those 12.5, 100 μg/mL groups (P<0. 05), and were similar to CSE 50 μg/mL group ( P > 0. 05 ). CONCLUSION: Ethyl acetate extract of cordyceps militaris may promote the HUVECs growth and proliferation, protecting from high glucose-stimulated vascular endothelial senescence. The effects may be associated with ethyl acetate extract of cordyceps militaris inhibiting reactive oxygen species and alleviating oxidative stress injury.关键词
人脐静脉内皮细胞/高糖/细胞衰老/抗氧化活性/蛹虫草乙酸乙酯提取物Key words
Human umbilical vein endothelial cells/ High glucose/ Cell senescence/ Antiox-idative activity/ Ethyl acetate extract of cordyceps militaris分类
医药卫生引用本文复制引用
朱鹏立,叶章正,余惠珍,黄华玮,阮景明,林帆..蛹虫草提取物对高糖诱导内皮细胞衰老的影响[J].中国临床药理学与治疗学,2012,17(11):1216-1222,7.基金项目
福建省医学创新课题(2011-CXB-3) （2011-CXB-3）
